ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAAALRSNTS RETASLTLSH FRYFIFNRIH TARTATSPPH CNHRSKSDEK FPYKISSLGT 

        70         80         90        100        110        120 
SFLDNRGGGE RRNSTKCYAA QKKLSGVGSS VVILSSQGDP PDLWQPPGDG VSVRVNGSSV 

       130        140        150        160        170        180 
NLGRGGGGGG SSPGGPGNGT GSNSKEDCWG GSNLGSDFPT PKEICKGLNK FVIGQERAKK 

       190        200        210        220        230        240 
VLSVAVYNHY KRIYHESSQK RSAGETDSTA AKPADDDMVE LEKSNILLMG PTGSGKTLLA 

       250        260        270        280        290        300 
KTLARFVNVP FVIADATTLT QAGYVGEDVE SILYKLLTVA DYNVAAAQQG IVYIDEVDKI 

       310        320        330        340        350        360 
TKKAESLNIS RDVSGEGVQQ ALLKMLEGTI VNVPEKGARK HPRGDNIQID TKDILFICGG 

       370        380        390        400        410        420 
AFVDIEKTIS ERRHDSSIGF GAPVRANMRA GGVTNAAVAS NLMETVESSD LIAYGLIPEF 

       430        440        450        460        470        480 
VGRFPVLVSL SALTENQLMQ VLTEPKNALG KQYKKMYQMN SVKLHFTESA LRLIARKAIT 

       490        500        510        520        530        540 
KNTGARGLRA LLESILMDSM YEIPDEGTGS DMIEAVVVDE EAVEGEGRRG SGAKILRGKG 

       550        560        570 
ALARYLSETN SKDSPQTTKE GSDGETEVEA EIPSVVASM 

References and documentation are available.

Number of amino acids: 579

Molecular weight: 61963.97

Theoretical pI: 7.62

Amino acid composition: 
Ala (A)  48	  8.3%
Arg (R)  31	  5.4%
Asn (N)  26	  4.5%
Asp (D)  29	  5.0%
Cys (C)   5	  0.9%
Gln (Q)  16	  2.8%
Glu (E)  39	  6.7%
Gly (G)  60	 10.4%
His (H)   9	  1.6%
Ile (I)  31	  5.4%
Leu (L)  46	  7.9%
Lys (K)  38	  6.6%
Met (M)  13	  2.2%
Phe (F)  15	  2.6%
Pro (P)  23	  4.0%
Ser (S)  56	  9.7%
Thr (T)  35	  6.0%
Trp (W)   2	  0.3%
Tyr (Y)  15	  2.6%
Val (V)  42	  7.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 68
Total number of positively charged residues (Arg + Lys): 69

Atomic composition:

Carbon      C	      2694
Hydrogen    H	      4358
Nitrogen    N	       772
Oxygen      O	       864
Sulfur      S	        18

Formula: C2694H4358N772O864S18
Total number of atoms: 8706

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    33600
Abs 0.1% (=1 g/l)   0.542, assuming all pairs of Cys residues form cystines


Ext. coefficient    33350
Abs 0.1% (=1 g/l)   0.538, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.63
This classifies the protein as stable.



Aliphatic index: 81.19

Grand average of hydropathicity (GRAVY): -0.340