ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAAAPFLKHW RTTFERVEKF VSPIYFTDCN LRGRLFGDSC PVTLSSFLTP ERLPYEKAVQ 

        70         80         90        100        110        120 
QNFSPAQVGD SFGPTWWTCW FRVELVIPEV WVGKEVHLCW ESDGESLVWR DGEPVQGLTK 

       130        140        150        160        170        180 
EGEKTSYVLS ERLHAADPRS LTLYVEVACN GLLGAGKGSM IAAPDPEKMF QLSQAKLAVF 

       190        200        210        220        230        240 
HRDVHNLLVD LELLLGVAKG LGEDNQRSFQ ALYTANQMVN ICDPAQPETY PAAEALASKF 

       250        260        270        280        290        300 
FGQRGGESQH TIHATGHCHI DTAWLWPFKE TVRKCARSWS TAVKLMERNT EFTFACSQAQ 

       310        320        330        340        350        360 
QLEWVKNQYP GLYAQLQEFA CRGQFVPVGG TWVEMDGNLP SGEAMVRQFL QGQNFFLQEF 

       370        380        390        400        410        420 
GKMCSEFWLP DTFGYSAQLP QIMQGCGIKR FLTQKLSWNL VNSFPHHTFF WEGLDGSQVL 

       430        440        450        460        470        480 
VHFPPGDSYG MQGSVEEVLK TVTNNRDKGR TNHSGFLFGF GDGGGGPTQT MLDRLKRLGN 

       490        500        510        520        530        540 
TDGLPRVQLS SPGQLFTALE RDSGQLCTWV GELFLELHNG TYTTHAQLKK GNRECEQILH 

       550        560        570        580        590        600 
DVELLSSLAL ARSAQFLYPA VQLQRLWRLL LLNQFHDVVT GSCIQLVAED AMNYYEDIRS 

       610        620        630        640        650        660 
HGNTLLSAAA AALCAGEPGP KGLLIINTLP WKRTEVLALP KPCGAHSLAL VTVPSIGYAP 

       670        680        690        700        710        720 
APTPTSLQPL LPQQPVFVMQ ETDGSVTLDN GIIRVRLDPT GCLTSLVLVA SGREAIAEGA 

       730        740        750        760        770        780 
LGNQFVLFDD VPLYWDAWDV MDYHLETRKP VRGQAGTLAV GAEGGLRGSA WFLLQISPNS 

       790        800        810        820        830        840 
RLSQEVVLDV GCPYVRFHTE VHWHETHKFL KVEFPARVRS PQATYEIQFG HLQRPTHNNT 

       850        860        870        880        890        900 
SWDWARYEVW AHRWIDLSEC DFGLALLNNC KYGTSVRGNV LSLSLLRAPK APDVTADMGR 

       910        920        930        940        950        960 
HEFTYALMPH KGSFQEAGVI QAAYNLNFPL LALPAPGPAP DTTWSAFSVS SPAVVLETIK 

       970        980        990       1000       1010       1020 
QAEKSHQHRT LVLRLYEAHG SHVDCWLHTS LPVQEATLCD LLEQRDPTGH LSLQDNRLKL 

      1030 
TFSPFQVRSL LLVLQPPAN 

References and documentation are available.

Number of amino acids: 1039

Molecular weight: 115839.93

Theoretical pI: 6.01

Amino acid composition: 
Ala (A)  80	  7.7%
Arg (R)  51	  4.9%
Asn (N)  35	  3.4%
Asp (D)  44	  4.2%
Cys (C)  23	  2.2%
Gln (Q)  60	  5.8%
Glu (E)  61	  5.9%
Gly (G)  81	  7.8%
His (H)  35	  3.4%
Ile (I)  22	  2.1%
Leu (L) 129	 12.4%
Lys (K)  35	  3.4%
Met (M)  16	  1.5%
Phe (F)  52	  5.0%
Pro (P)  62	  6.0%
Ser (S)  64	  6.2%
Thr (T)  63	  6.1%
Trp (W)  28	  2.7%
Tyr (Y)  24	  2.3%
Val (V)  74	  7.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 105
Total number of positively charged residues (Arg + Lys): 86

Atomic composition:

Carbon      C	      5220
Hydrogen    H	      8004
Nitrogen    N	      1420
Oxygen      O	      1496
Sulfur      S	        39

Formula: C5220H8004N1420O1496S39
Total number of atoms: 16179

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   191135
Abs 0.1% (=1 g/l)   1.650, assuming all pairs of Cys residues form cystines


Ext. coefficient   189760
Abs 0.1% (=1 g/l)   1.638, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.19
This classifies the protein as stable.



Aliphatic index: 85.03

Grand average of hydropathicity (GRAVY): -0.177