ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAAAPFLKHW RTTFERVEKF VSPIYFTDCN LRGRLFGDSC SVTLSSFLTP ERLPYEKAVQ 

        70         80         90        100        110        120 
QNFSPAQVGD SFGPTWWTCW FRVELVIPEV WVGQEVHLCW ESDGESLVWR DGEPVQGLTK 

       130        140        150        160        170        180 
EGEKTSYVLS ERLRASDPRS LTLYVEVACN GLLGAGKGSM IAAPDPEKMF QLSQAKLAVF 

       190        200        210        220        230        240 
HRDVHSLLVD LELLLGVAKG LGEDSQRSFQ ALHTANQMVN ICDPAQPETY PAAKALASKF 

       250        260        270        280        290        300 
FGQHGGESQH TIHAMGHCHI DTAWLWPFKE TVRKCARSWS TAVTLMEQNT DFIFACSQAQ 

       310        320        330        340        350        360 
QLEWVKSQYP GLHARLQEFA CRGQFVPVGG TWVEMDGNLP SGEAMVRQFL QGQNFFLQEF 

       370        380        390        400        410        420 
GKMCSEFWLP DTFGYSAQLP QIMQGCGIKR FLTQKLSWNL VNSFPHHTFF WEGLDGSRVL 

       430        440        450        460        470        480 
VHFPPGDSYG MQGSVEEVLK TVTNNRDKGR TNHSGFLFGF GDGGGGPTQT MLDRLKRLSN 

       490        500        510        520        530        540 
TDGLPRVQLS SPGQLFTALE RDSGQLCTWV GELFLELHNG TYTTHAQLKK GNRECEQILH 

       550        560        570        580        590        600 
DVEVLSSLAL ARSAQFLYPA AQLQHLWRLL LLNQFHDVVT GSCIQLVAED AMNYYEDIRS 

       610        620        630        640        650        660 
HGNPLLSAAA AALCAGEPGP KGLLIINTLP WKRTEVLALP KPCGAHSLAL VTVPSIGYAP 

       670        680        690        700        710        720 
APTPTSLQPL LPQQPVFVMQ ETDGSVTLDN GIIRVRLDPT GCLTSLVLVA SGREAIAEGA 

       730        740        750        760        770        780 
LGNQFVLFDD VPLYWDAWDV MDYHLETRKP VLGQAGTLAV GTEGGLRGSA WFLLQISPNS 

       790        800        810        820        830        840 
RLSQEVVLDV GCPYVRFHTE VHWHEAHKFL KVEFPARIRS PQATYEIQFG HLQRPTHNNT 

       850        860        870        880        890        900 
SWDWARYEVW AHRWIDLSEC DFGLALLNNC KYGASVRGNV LSLSLLRAPK APDATADMGR 

       910        920        930        940        950        960 
HEFTYALMPH KGSFQEAGVI HAAYNLNFPL LALPAPGPAP DTTWSAFSVS SPAVVLETIK 

       970        980        990       1000       1010       1020 
QAERCHQHRT LVLRLYEAHG SHVDCWLHTS LPVQEATLCD LLEQRDPTGH LSLQDNRLKL 

      1030 
TFSPFQVRSL LLVLQSPPN 

References and documentation are available.

Number of amino acids: 1039

Molecular weight: 115687.78

Theoretical pI: 6.04

Amino acid composition: 
Ala (A)  81	  7.8%
Arg (R)  51	  4.9%
Asn (N)  32	  3.1%
Asp (D)  45	  4.3%
Cys (C)  24	  2.3%
Gln (Q)  59	  5.7%
Glu (E)  59	  5.7%
Gly (G)  80	  7.7%
His (H)  39	  3.8%
Ile (I)  24	  2.3%
Leu (L) 129	 12.4%
Lys (K)  33	  3.2%
Met (M)  17	  1.6%
Phe (F)  52	  5.0%
Pro (P)  62	  6.0%
Ser (S)  70	  6.7%
Thr (T)  60	  5.8%
Trp (W)  28	  2.7%
Tyr (Y)  22	  2.1%
Val (V)  72	  6.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 104
Total number of positively charged residues (Arg + Lys): 84

Atomic composition:

Carbon      C	      5208
Hydrogen    H	      7984
Nitrogen    N	      1422
Oxygen      O	      1491
Sulfur      S	        41

Formula: C5208H7984N1422O1491S41
Total number of atoms: 16146

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   188280
Abs 0.1% (=1 g/l)   1.627, assuming all pairs of Cys residues form cystines


Ext. coefficient   186780
Abs 0.1% (=1 g/l)   1.615, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.77
This classifies the protein as unstable.



Aliphatic index: 85.32

Grand average of hydropathicity (GRAVY): -0.158