ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAAEKKVFVI FDHQEQRHDQ PWPSYHIEVP RRLDAILERL NTTKLLTDPR IEHIPRREAE 

        70         80         90        100        110        120 
ESEILAVHTK RYVDDVKSTE TMTVEQQESF CTKYEDIYVN SATWHRAKLA AGASIDLMTS 

       130        140        150        160        170        180 
VMAAKRPGIA FIRPPGHHAM PDEGCGFCIF NNVAIAAKAA IQNGQKVLIV DYDVHAGNGT 

       190        200        210        220        230        240 
QECVEQMGEG NVQLISIHRY ENGHFWPNMP QTGIYHNYKN TINLPLNTIG LTDADYHALF 

       250        260        270        280        290        300 
THIILPTIHA FQPDLLLVSS GFDASIGDPE GSMQVTPAGF ATMIRMLIDT GIPVAALLEG 

       310        320        330        340        350        360 
GYFLDALAAD SEWVLRALLG EEIPRIRVEK IHSAIADTIG RVVKRYEGSC PFFKKVQELR 

       370        380        390        400        410        420 
GILGCRVAEE DQQEEATDYT GERTVHPPYE TRGIYTPFTE QKVAEFKDQL ESILASYTTP 

       430        440        450        460        470        480 
PDFSAETEAF HSAIAPHSSE INDFYFGVHE DKILLGTSPA VRLFFELLFT APYDPKHTIK 

       490        500        510 
LYGKVKPEDF GKIELDSRSL GEMKNISIFN NLPIFQL 

References and documentation are available.

Number of amino acids: 517

Molecular weight: 58090.97

Theoretical pI: 5.31

Amino acid composition: 
Ala (A)  44	  8.5%
Arg (R)  24	  4.6%
Asn (N)  17	  3.3%
Asp (D)  29	  5.6%
Cys (C)   6	  1.2%
Gln (Q)  19	  3.7%
Glu (E)  43	  8.3%
Gly (G)  32	  6.2%
His (H)  21	  4.1%
Ile (I)  42	  8.1%
Leu (L)  40	  7.7%
Lys (K)  24	  4.6%
Met (M)  11	  2.1%
Phe (F)  26	  5.0%
Pro (P)  30	  5.8%
Ser (S)  25	  4.8%
Thr (T)  33	  6.4%
Trp (W)   4	  0.8%
Tyr (Y)  18	  3.5%
Val (V)  29	  5.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 72
Total number of positively charged residues (Arg + Lys): 48

Atomic composition:

Carbon      C	      2611
Hydrogen    H	      4035
Nitrogen    N	       695
Oxygen      O	       774
Sulfur      S	        17

Formula: C2611H4035N695O774S17
Total number of atoms: 8132

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    49195
Abs 0.1% (=1 g/l)   0.847, assuming all pairs of Cys residues form cystines


Ext. coefficient    48820
Abs 0.1% (=1 g/l)   0.840, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.52
This classifies the protein as unstable.



Aliphatic index: 86.63

Grand average of hydropathicity (GRAVY): -0.246