ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAAGGQPQGA TPGQPDQNFD YMFKLLIIGN SSVGKTSFLF RYCDDSFTSA FVSTVGIDFK 

        70         80         90        100        110        120 
VKTVFRGDKR VKLQIWDTAG QERYRTITTA YYRGAMGFIL MYDITNEESF NSVQDWCTQI 

       130        140        150        160        170        180 
KTYSWENAQV VLVGNKCDMD SERVVSMDRG RQLADQLGLE FFETSAKENI NVKAVFEKLV 

       190        200        210 
EIICDKMAES LDKDPQQQPK GQKLEANPTQ KPAQQQCNC 

References and documentation are available.

Number of amino acids: 219

Molecular weight: 24760.00

Theoretical pI: 5.18

Amino acid composition: 
Ala (A)  14	  6.4%
Arg (R)   9	  4.1%
Asn (N)  10	  4.6%
Asp (D)  16	  7.3%
Cys (C)   6	  2.7%
Gln (Q)  19	  8.7%
Glu (E)  12	  5.5%
Gly (G)  15	  6.8%
His (H)   0	  0.0%
Ile (I)  11	  5.0%
Leu (L)  12	  5.5%
Lys (K)  16	  7.3%
Met (M)   7	  3.2%
Phe (F)  13	  5.9%
Pro (P)   7	  3.2%
Ser (S)  13	  5.9%
Thr (T)  14	  6.4%
Trp (W)   3	  1.4%
Tyr (Y)   7	  3.2%
Val (V)  15	  6.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 28
Total number of positively charged residues (Arg + Lys): 25

Atomic composition:

Carbon      C	      1090
Hydrogen    H	      1696
Nitrogen    N	       294
Oxygen      O	       339
Sulfur      S	        13

Formula: C1090H1696N294O339S13
Total number of atoms: 3432

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    27305
Abs 0.1% (=1 g/l)   1.103, assuming all pairs of Cys residues form cystines


Ext. coefficient    26930
Abs 0.1% (=1 g/l)   1.088, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 30.59
This classifies the protein as stable.



Aliphatic index: 67.21

Grand average of hydropathicity (GRAVY): -0.473