ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAAHQMDSIT YSNNVTQQYI QPQSLQDISA VEDEIQNKIE AARQESKQLH AQINKAKHKI 

        70         80         90        100        110        120 
QDASLFQMAN KVTSLTKNKI NLKPNIVLKG HNNKISDFRW SRDSKRILSA SQDGFMLIWD 

       130        140        150        160        170        180 
SASGLKQNAI PLDSQWVLSC AISPSSTLVA SAGLNNNCTI YRVSKENRVA QNVASIFKGH 

       190        200        210        220        230        240 
TCYISDIEFT DNAHILTASG DMTCALWDIP KAKRVREYSD HLGDVLALAI PEEPNSENSS 

       250        260        270        280        290        300 
NTFASCGSDG YTYIWDSRSP SAVQSFYVND SDINALRFFK DGMSIVAGSD NGAINMYDLR 

       310        320        330        340        350        360 
SDCSIATFSL FRGYEERTPT PTYMAANMEY NTAQSPQTLK STSSSYLDNQ GVVSLDFSAS 

       370        380        390        400        410        420 
GRLMYSCYTD IGCVVWDVLK GEIVGKLEGH GGRVTGVRSS PDGLAVCTGS WDSTMKIWSP 


GYQ 

References and documentation are available.

Number of amino acids: 423

Molecular weight: 46581.10

Theoretical pI: 5.79

Amino acid composition: 
Ala (A)  34	  8.0%
Arg (R)  16	  3.8%
Asn (N)  27	  6.4%
Asp (D)  29	  6.9%
Cys (C)   9	  2.1%
Gln (Q)  21	  5.0%
Glu (E)  15	  3.5%
Gly (G)  25	  5.9%
His (H)   8	  1.9%
Ile (I)  29	  6.9%
Leu (L)  28	  6.6%
Lys (K)  22	  5.2%
Met (M)  11	  2.6%
Phe (F)  12	  2.8%
Pro (P)  13	  3.1%
Ser (S)  54	 12.8%
Thr (T)  23	  5.4%
Trp (W)   8	  1.9%
Tyr (Y)  16	  3.8%
Val (V)  23	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 44
Total number of positively charged residues (Arg + Lys): 38

Atomic composition:

Carbon      C	      2030
Hydrogen    H	      3171
Nitrogen    N	       565
Oxygen      O	       653
Sulfur      S	        20

Formula: C2030H3171N565O653S20
Total number of atoms: 6439

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    68340
Abs 0.1% (=1 g/l)   1.467, assuming all pairs of Cys residues form cystines


Ext. coefficient    67840
Abs 0.1% (=1 g/l)   1.456, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 52.31
This classifies the protein as unstable.



Aliphatic index: 76.36

Grand average of hydropathicity (GRAVY): -0.359