ProtParam
User-provided sequence:
10 20 30 40 50 60
MAAHQMDSIT YSNNVTQQYI QPQSLQDISA VEDEIQNKIE AARQESKQLH AQINKAKHKI
70 80 90 100 110 120
QDASLFQMAN KVTSLTKNKI NLKPNIVLKG HNNKISDFRW SRDSKRILSA SQDGFMLIWD
130 140 150 160 170 180
SASGLKQNAI PLDSQWVLSC AISPSSTLVA SAGLNNNCTI YRVSKENRVA QNVASIFKGH
190 200 210 220 230 240
TCYISDIEFT DNAHILTASG DMTCALWDIP KAKRVREYSD HLGDVLALAI PEEPNSENSS
250 260 270 280 290 300
NTFASCGSDG YTYIWDSRSP SAVQSFYVND SDINALRFFK DGMSIVAGSD NGAINMYDLR
310 320 330 340 350 360
SDCSIATFSL FRGYEERTPT PTYMAANMEY NTAQSPQTLK STSSSYLDNQ GVVSLDFSAS
370 380 390 400 410 420
GRLMYSCYTD IGCVVWDVLK GEIVGKLEGH GGRVTGVRSS PDGLAVCTGS WDSTMKIWSP
GYQ
References and
documentation are available.
Number of amino acids: 423
Molecular weight: 46581.10
Theoretical pI: 5.79
Total number of negatively charged residues (Asp + Glu): 44
Total number of positively charged residues (Arg + Lys): 38
Atomic composition:
Carbon C 2030
Hydrogen H 3171
Nitrogen N 565
Oxygen O 653
Sulfur S 20
Formula: C
2030H
3171N
565O
653S
20
Total number of atoms: 6439
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 68340
Abs 0.1% (=1 g/l) 1.467, assuming all pairs of Cys residues form cystines
Ext. coefficient 67840
Abs 0.1% (=1 g/l) 1.456, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 52.31
This classifies the protein as unstable.
Aliphatic index: 76.36
Grand average of hydropathicity (GRAVY): -0.359