ProtParam
User-provided sequence:
10 20 30 40 50 60
MAAIRKKLVI VGDGACGKTC LLIVFSKDQF PDVYVPTVFE NYVADIEVDG KQVELALWDT
70 80 90 100 110 120
AGQEDYDRLR PLSYPDTDVI LMCFSIDSPD SLENIPEKWT PEVRHFCPNV PIILVGNKRD
130 140 150 160 170 180
LRSDPQTVRE LAKMKQEPVK PEQGRAIAEQ IGAFAYLECS AKTKDGIREV FEKATQAALQ
190
QKKKKKSKCM IL
References and
documentation are available.
Number of amino acids: 192
Molecular weight: 21635.07
Theoretical pI: 5.97
Total number of negatively charged residues (Asp + Glu): 29
Total number of positively charged residues (Arg + Lys): 28
Atomic composition:
Carbon C 965
Hydrogen H 1550
Nitrogen N 256
Oxygen O 286
Sulfur S 10
Formula: C
965H
1550N
256O
286S
10
Total number of atoms: 3067
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 18825
Abs 0.1% (=1 g/l) 0.870, assuming all pairs of Cys residues form cystines
Ext. coefficient 18450
Abs 0.1% (=1 g/l) 0.853, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 50.90
This classifies the protein as unstable.
Aliphatic index: 88.85
Grand average of hydropathicity (GRAVY): -0.310