ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAAIRKKLVI VGDGACGKTC LLIVFSKDQF PDVYVPTVFE NYVADIEVDG KQVELALWDT 

        70         80         90        100        110        120 
AGQEDYDRLR PLSYPDTDVI LMCFSIDSPD SLENIPEKWT PEVRHFCPNV PIILVGNKRD 

       130        140        150        160        170        180 
LRSDPQTVRE LAKMKQEPVK PEQGRAIAEQ IGAFAYLECS AKTKDGIREV FEKATQAALQ 

       190 
QKKKKKSKCM IL 

References and documentation are available.

Number of amino acids: 192

Molecular weight: 21635.07

Theoretical pI: 5.97

Amino acid composition: 
Ala (A)  15	  7.8%
Arg (R)   9	  4.7%
Asn (N)   4	  2.1%
Asp (D)  15	  7.8%
Cys (C)   6	  3.1%
Gln (Q)  10	  5.2%
Glu (E)  14	  7.3%
Gly (G)   9	  4.7%
His (H)   1	  0.5%
Ile (I)  13	  6.8%
Leu (L)  15	  7.8%
Lys (K)  19	  9.9%
Met (M)   4	  2.1%
Phe (F)   7	  3.6%
Pro (P)  12	  6.2%
Ser (S)   8	  4.2%
Thr (T)   8	  4.2%
Trp (W)   2	  1.0%
Tyr (Y)   5	  2.6%
Val (V)  16	  8.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 29
Total number of positively charged residues (Arg + Lys): 28

Atomic composition:

Carbon      C	       965
Hydrogen    H	      1550
Nitrogen    N	       256
Oxygen      O	       286
Sulfur      S	        10

Formula: C965H1550N256O286S10
Total number of atoms: 3067

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    18825
Abs 0.1% (=1 g/l)   0.870, assuming all pairs of Cys residues form cystines


Ext. coefficient    18450
Abs 0.1% (=1 g/l)   0.853, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.90
This classifies the protein as unstable.



Aliphatic index: 88.85

Grand average of hydropathicity (GRAVY): -0.310