ProtParam
User-provided sequence:
10 20 30 40 50 60
MAAKHEYNKL SLVHLPRKEV HSEKRIPSIL TRTIAVLFVF ALLALVSIGS EGVPQLRNSR
70 80 90 100 110 120
DLTKSPCKKR VVGYYSEWEG TEITRSQLGK LTHAVFAFIH MDSEGKLQFK TNQKERFEKL
130 140 150 160 170 180
KTAVKNANSD TKVMISIGGD HNSENFGSVL SDSEKKSMFI DSIARFIRQH KIHGVDIYWK
190 200 210 220 230 240
WLGNSETEHH DFPSFLKDLK EKLKTVRDDS IISIVAPQAK MDRRHDGYKF DDFMEYIDFV
250 260 270 280 290 300
NVFSMDYYGP WPNQWGTPTG PSAPLYGGIG VKKHFNVDST MKYYTCMTED PSKFNMVIPF
310 320 330 340 350 360
YVRLWKNVKE PISSGTEVFR RADLKNGAAV GNSYMSRWTV DHEGWELTPA LWDDVTKTPY
370 380 390 400 410 420
VWNQETGNFL TFENKKSIEA KLAYAIEHNL GGVWIHLVDK DNENEELLRA VASKKFCASE
SENKVTYDC
References and
documentation are available.
Number of amino acids: 429
Molecular weight: 49240.01
Theoretical pI: 8.12
Total number of negatively charged residues (Asp + Glu): 56
Total number of positively charged residues (Arg + Lys): 58
Atomic composition:
Carbon C 2220
Hydrogen H 3412
Nitrogen N 594
Oxygen O 646
Sulfur S 15
Formula: C
2220H
3412N
594O
646S
15
Total number of atoms: 6887
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 84590
Abs 0.1% (=1 g/l) 1.718, assuming all pairs of Cys residues form cystines
Ext. coefficient 84340
Abs 0.1% (=1 g/l) 1.713, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 40.50
This classifies the protein as unstable.
Aliphatic index: 74.50
Grand average of hydropathicity (GRAVY): -0.500