ProtParam
User-provided sequence:
10 20 30 40 50 60
MAAKKVFGSA EASNLVTELR RSFDDGVTRG YEWRVTQLKK LMIICDNHEP EIVAALRDDL
70 80 90 100 110 120
GKPELESSVY EVSLLRNSIK LALKQLKNWM APEKAKTSLT TFPASAEIVS EPLGVVLVIS
130 140 150 160 170 180
AWNYPFLLSI DPVIGAISAG NAVVLKPSEL APASSALLTK LLEQYLDPSA VRVVEGAVTE
190 200 210 220 230 240
TSALLEQKWD KIFYTGSSKI GRVIMAAAAK HLTPVVLELG GKSPVVVDSD TDLKVTVRRI
250 260 270 280 290 300
IVGKWGCNNG QACVSPDYIL TTKEYAPKLI DAMKLELEKF YGKNPIESKD MSRIVNSNHF
310 320 330 340 350 360
DRLSKLLDEK EVSDKIVYGG EKDRENLKIA PTILLDVPLD SLIMSEEIFG PLLPILTLNN
370 380 390 400 410 420
LEESFDVIRS RPKPLAAYLF THNKKLKERF AATVSAGGIV VNDIAVHLAL HTLPFGGVGE
430 440 450 460 470 480
SGMGAYHGKF SFDAFSHKKA VLYRSLFGDS AVRYPPYSRG KLRLLKALVD SNIFDLFKVL
LGLA
References and
documentation are available.
Number of amino acids: 484
Molecular weight: 53158.80
Theoretical pI: 8.43
Total number of negatively charged residues (Asp + Glu): 57
Total number of positively charged residues (Arg + Lys): 60
Atomic composition:
Carbon C 2414
Hydrogen H 3879
Nitrogen N 627
Oxygen O 695
Sulfur S 11
Formula: C
2414H
3879N
627O
695S
11
Total number of atoms: 7626
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 48485
Abs 0.1% (=1 g/l) 0.912, assuming all pairs of Cys residues form cystines
Ext. coefficient 48360
Abs 0.1% (=1 g/l) 0.910, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.04
This classifies the protein as stable.
Aliphatic index: 107.17
Grand average of hydropathicity (GRAVY): 0.048