ProtParam
User-provided sequence:
10 20 30 40 50 60
MAALKLLSSG LRLCASARNS RGALHKGCAC YFSVSTRHHT KFYTDPVEAV KDIPNGATLL
70 80 90 100 110 120
VGGFGLCGIP ENLIGALLKT GVKDLTAVSN NAGVDNFGLG LLLRSKQIKR MISSYVGENA
130 140 150 160 170 180
EFERQFLSGE LEVELTPQGT LAERIRAGGA GVPAFYTSTG YGTLVQEGGS PIKYNKDGSV
190 200 210 220 230 240
AIASKPREVR EFRGQHFILE EAITGDFALV KAWKADRAGN VIFRKSARNF NLPMCKAAGT
250 260 270 280 290 300
TVVEVEEIVD IGSFAPEDIH IPKIYVHRLI KGEKYEKRIE RLSLRKEGEG KAKSGKPGED
310 320 330 340 350 360
VRERIIKRAA LEFEDGMYAN LGIGIPLLAS NFISPNMTVH LQSENGVLGL GPYPLKDEAD
370 380 390 400 410 420
ADLINAGKET VTVLPGASFF SSDESFAMIR GGHVNLTMLG AMQVSKYGDL ANWMIPGKMV
430 440 450 460 470 480
KGMGGAMDLV SSSKTKVVVT MEHSAKGNAH KIMEKCTLPL TGKQCVNRII TEKGVFDVDK
490 500 510 520
KNGLTLIELW EGLTVDDIRK STGCDFAVSP NLMPMQQIST
References and
documentation are available.
Number of amino acids: 520
Molecular weight: 56203.84
Theoretical pI: 8.70
Total number of negatively charged residues (Asp + Glu): 58
Total number of positively charged residues (Arg + Lys): 64
Atomic composition:
Carbon C 2488
Hydrogen H 4013
Nitrogen N 689
Oxygen O 741
Sulfur S 24
Formula: C
2488H
4013N
689O
741S
24
Total number of atoms: 7955
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 33390
Abs 0.1% (=1 g/l) 0.594, assuming all pairs of Cys residues form cystines
Ext. coefficient 32890
Abs 0.1% (=1 g/l) 0.585, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 26.52
This classifies the protein as stable.
Aliphatic index: 89.65
Grand average of hydropathicity (GRAVY): -0.115