ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAALVLSLLL LSLAGHSSAS WCVCKTGLSD TVLQATLDYA CGNGADCNPT KPKQSCFNPD 

        70         80         90        100        110        120 
NVRSHCNYAV NSFFQKKGQS PGSCNFDGTA TPTNSDPSYT GCAFPTSASG SSGSTTVTPG 

       130        140        150        160        170        180 
TTNPKGSPTT TTLPGSGTNS PYSGNPTNGV FGGNSTGGTT GTGINPDYTT DSSAFALKNS 

       190        200 
SKLFICLLLI ASSGFCSFLM L 

References and documentation are available.

Number of amino acids: 201

Molecular weight: 20364.58

Theoretical pI: 7.47

Amino acid composition: 
Ala (A)  14	  7.0%
Arg (R)   1	  0.5%
Asn (N)  15	  7.5%
Asp (D)   8	  4.0%
Cys (C)  10	  5.0%
Gln (Q)   4	  2.0%
Glu (E)   0	  0.0%
Gly (G)  23	 11.4%
His (H)   2	  1.0%
Ile (I)   3	  1.5%
Leu (L)  18	  9.0%
Lys (K)   8	  4.0%
Met (M)   2	  1.0%
Phe (F)  10	  5.0%
Pro (P)  14	  7.0%
Ser (S)  30	 14.9%
Thr (T)  26	 12.9%
Trp (W)   1	  0.5%
Tyr (Y)   5	  2.5%
Val (V)   7	  3.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 8
Total number of positively charged residues (Arg + Lys): 9

Atomic composition:

Carbon      C	       877
Hydrogen    H	      1362
Nitrogen    N	       236
Oxygen      O	       298
Sulfur      S	        12

Formula: C877H1362N236O298S12
Total number of atoms: 2785

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    13575
Abs 0.1% (=1 g/l)   0.667, assuming all pairs of Cys residues form cystines


Ext. coefficient    12950
Abs 0.1% (=1 g/l)   0.636, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 27.78
This classifies the protein as stable.



Aliphatic index: 57.81

Grand average of hydropathicity (GRAVY): -0.122