ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAAMNPEYDY LFKLLLIGDS GVGKSCLLLR FADDTYTESY ISTIGVDFKI RTIELDGKTI 

        70         80         90        100        110        120 
KLQIWDTAGQ ERFRTITSSY YRGAHGIIVV YDITDQETFN NVKQWLQEID RYACENVNKL 

       130        140        150        160        170        180 
LVGNKCDLTA KRAVETQAAQ DYAGQLGIPF LETSAKSSTN VEQAFLTMAS EIKSRMGPVQ 

       190        200 
GAGGAPGVRI TGSQPVQDKK SGGCC 

References and documentation are available.

Number of amino acids: 205

Molecular weight: 22545.60

Theoretical pI: 5.52

Amino acid composition: 
Ala (A)  16	  7.8%
Arg (R)   9	  4.4%
Asn (N)   7	  3.4%
Asp (D)  13	  6.3%
Cys (C)   5	  2.4%
Gln (Q)  12	  5.9%
Glu (E)  11	  5.4%
Gly (G)  19	  9.3%
His (H)   1	  0.5%
Ile (I)  15	  7.3%
Leu (L)  16	  7.8%
Lys (K)  13	  6.3%
Met (M)   4	  2.0%
Phe (F)   7	  3.4%
Pro (P)   5	  2.4%
Ser (S)  13	  6.3%
Thr (T)  16	  7.8%
Trp (W)   2	  1.0%
Tyr (Y)   9	  4.4%
Val (V)  12	  5.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 24
Total number of positively charged residues (Arg + Lys): 22

Atomic composition:

Carbon      C	       994
Hydrogen    H	      1576
Nitrogen    N	       268
Oxygen      O	       311
Sulfur      S	         9

Formula: C994H1576N268O311S9
Total number of atoms: 3158

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    24660
Abs 0.1% (=1 g/l)   1.094, assuming all pairs of Cys residues form cystines


Ext. coefficient    24410
Abs 0.1% (=1 g/l)   1.083, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.78
This classifies the protein as stable.



Aliphatic index: 83.76

Grand average of hydropathicity (GRAVY): -0.236