ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAANFWNSSH YKQLLDPEEV DVVHDLDKER GISIDDFKLI KFHMSNHIMK LAQHIKVRQR 

        70         80         90        100        110        120 
VVATAITYMR RVYIRKSMVE FEPRLVALTC LYLASKAEES IVQARNLVFY IKRLYPDEYN 

       130        140        150        160        170        180 
KYELKDILGM EMKVLEALDY YLVVFHPYRS LSEFLQDAAL NDVNMNQITW GIVNDTYKMD 

       190        200        210        220        230        240 
LILVHPPYRI ALACIYIASV HREKDITAWF EDLHEDMNLV KNIAMEILDF YENYRTITEE 

       250 
KVNSAFSKLA LKL 

References and documentation are available.

Number of amino acids: 253

Molecular weight: 29914.74

Theoretical pI: 6.00

Amino acid composition: 
Ala (A)  19	  7.5%
Arg (R)  13	  5.1%
Asn (N)  13	  5.1%
Asp (D)  17	  6.7%
Cys (C)   2	  0.8%
Gln (Q)   6	  2.4%
Glu (E)  19	  7.5%
Gly (G)   3	  1.2%
His (H)   9	  3.6%
Ile (I)  20	  7.9%
Leu (L)  28	 11.1%
Lys (K)  18	  7.1%
Met (M)  11	  4.3%
Phe (F)  10	  4.0%
Pro (P)   6	  2.4%
Ser (S)  12	  4.7%
Thr (T)   8	  3.2%
Trp (W)   3	  1.2%
Tyr (Y)  16	  6.3%
Val (V)  20	  7.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 31

Atomic composition:

Carbon      C	      1362
Hydrogen    H	      2124
Nitrogen    N	       350
Oxygen      O	       381
Sulfur      S	        13

Formula: C1362H2124N350O381S13
Total number of atoms: 4230

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    40465
Abs 0.1% (=1 g/l)   1.353, assuming all pairs of Cys residues form cystines


Ext. coefficient    40340
Abs 0.1% (=1 g/l)   1.348, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.39
This classifies the protein as stable.



Aliphatic index: 104.43

Grand average of hydropathicity (GRAVY): -0.123