ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAANSGLDSK VEYYRLQENE IISAVSSEDA DQNDAGFRLS TIHLHLFHGL KFAALLFTVV 

        70         80         90        100        110        120 
PVFIILDSMK IIFQRKRRFC LDHVNRSFLR QSSWILDERI CQYVLNPLFV CLYPSTFSSP 

       130        140        150        160        170        180 
TYVKCNIPIE DQKSPENNIF QDHQLNAPKI VSTKFYQYVM PEGFDPTTDP VLVFYHGGGY 

       190        200        210        220        230        240 
ALKLTPTSFS FLNNMRNAFP KMAILVPDYT VTATDDQSKK YPLQILQNVA IFDYVVKTMG 

       250        260        270        280        290        300 
CKNVVIMGDS AGGNAVLNIV LYLRKCHREI YPKKVIAISP WANATFFHEG EKEYMQGTQE 

       310        320        330        340        350        360 
WDGLCLKSHS MFGRMFVGNN PNVDFTSDPF VNIEKNFETK MWQDILKKCS VMITYGSDEL 

       370        380        390        400        410        420 
LSFQNKILAK KMSDASEGCN HFTAKNVLVE HQGYHTGPIL NYSRNMDRWT NIPSIARILE 


FMQS 

References and documentation are available.

Number of amino acids: 424

Molecular weight: 48540.81

Theoretical pI: 7.17

Amino acid composition: 
Ala (A)  22	  5.2%
Arg (R)  15	  3.5%
Asn (N)  28	  6.6%
Asp (D)  23	  5.4%
Cys (C)   9	  2.1%
Gln (Q)  18	  4.2%
Glu (E)  19	  4.5%
Gly (G)  20	  4.7%
His (H)  12	  2.8%
Ile (I)  29	  6.8%
Leu (L)  35	  8.3%
Lys (K)  27	  6.4%
Met (M)  15	  3.5%
Phe (F)  28	  6.6%
Pro (P)  20	  4.7%
Ser (S)  31	  7.3%
Thr (T)  21	  5.0%
Trp (W)   5	  1.2%
Tyr (Y)  18	  4.2%
Val (V)  29	  6.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 42
Total number of positively charged residues (Arg + Lys): 42

Atomic composition:

Carbon      C	      2196
Hydrogen    H	      3371
Nitrogen    N	       571
Oxygen      O	       625
Sulfur      S	        24

Formula: C2196H3371N571O625S24
Total number of atoms: 6787

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    54820
Abs 0.1% (=1 g/l)   1.129, assuming all pairs of Cys residues form cystines


Ext. coefficient    54320
Abs 0.1% (=1 g/l)   1.119, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 52.68
This classifies the protein as unstable.



Aliphatic index: 83.89

Grand average of hydropathicity (GRAVY): -0.171