ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAAPDYALTD LIESDPRFES LKTRLAGYTK GSDEYIEELY SQLPLTSYPR YKTFLKKQAV 

        70         80         90        100        110        120 
AISNPDNEAG FSSIYRSSLS SENLVSCVDK NLRTAYDHFM FSARRWPQRD CLGSRPIDKA 

       130        140        150        160        170        180 
TGTWEETFRF ESYSTVSKRC HNIGSGILSL VNTKRKRPLE ANDFVVAILS HNNPEWILTD 

       190        200        210        220        230        240 
LACQAYSLTN TALYETLGPN TSEYILNLTE APILIFAKSN MYHVLKMVPD MKFVNTLVCM 

       250        260        270        280        290        300 
DELTHDELRM LNESLLPVKC NSLNEKITFF SLEQVEQVGC FNKIPAIPPT PDSLYTISFT 

       310        320        330        340        350        360 
SGTTGLPKGV EMSHRNIASG IAFAFSTFRI PPDKRNQQLY DMCFLPLAHI FERMVIAYDL 

       370        380        390        400        410        420 
AIGFGIGFLH KPDPTVLVED LKILKPYAVA LVPRILTRFE AGIKNALDKS TVQRNVANTI 

       430        440        450        460        470        480 
LDSKSARFTA RGGPDKSIMN FLVYHRVLID KIRDSLGLSN NSFIITGSAP ISKDTLLFLR 

       490        500        510        520        530        540 
SALDIGIRQG YGLTETFAGV CLSEPFEKDV GSCGAIGISA ECRLKSVPEM GYHADKDLKG 

       550        560        570        580        590        600 
ELQIRGPQVF ERYFKNPNET SKAVDQDGWF STGDVAFIDG KGRISVIDRV KNFFKLAHGE 

       610        620        630        640        650        660 
YIAPEKIENI YLSSCPYITQ IFVFGDPLKT FLVGIVGVDV DAAQPILAAK HPEVKTWTKE 

       670        680        690        700        710        720 
VLVENLNRNK KLRKEFLNKI NKCTDGLQGF EKLHNIKVGL EPLTLEDDVV TPTFKIKRAK 

       730        740 
ASKFFKDTLD QLYAEGSLVK TEKL 

References and documentation are available.

Number of amino acids: 744

Molecular weight: 83437.96

Theoretical pI: 7.82

Amino acid composition: 
Ala (A)  48	  6.5%
Arg (R)  35	  4.7%
Asn (N)  36	  4.8%
Asp (D)  43	  5.8%
Cys (C)  13	  1.7%
Gln (Q)  17	  2.3%
Glu (E)  45	  6.0%
Gly (G)  43	  5.8%
His (H)  13	  1.7%
Ile (I)  51	  6.9%
Leu (L)  78	 10.5%
Lys (K)  55	  7.4%
Met (M)  12	  1.6%
Phe (F)  42	  5.6%
Pro (P)  38	  5.1%
Ser (S)  54	  7.3%
Thr (T)  47	  6.3%
Trp (W)   5	  0.7%
Tyr (Y)  25	  3.4%
Val (V)  44	  5.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 88
Total number of positively charged residues (Arg + Lys): 90

Atomic composition:

Carbon      C	      3765
Hydrogen    H	      5930
Nitrogen    N	       988
Oxygen      O	      1100
Sulfur      S	        25

Formula: C3765H5930N988O1100S25
Total number of atoms: 11808

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    65500
Abs 0.1% (=1 g/l)   0.785, assuming all pairs of Cys residues form cystines


Ext. coefficient    64750
Abs 0.1% (=1 g/l)   0.776, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.74
This classifies the protein as stable.



Aliphatic index: 91.22

Grand average of hydropathicity (GRAVY): -0.172