ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAAQGYGYYR TVIFTAMFGG YSLYYFNRKT FSFVMPSLVD EIALDKDDLG LITSSQSAAY 

        70         80         90        100        110        120 
AISKFVSGVL SDQMSARWLF SSGLLLVGLV NVVFSWSSTV PAFAALWFLN GLAQGLGWPP 

       130        140        150        160        170        180 
CGKILRKWFE PSQFGTWWAV LSTSMNLAGS LGPILATILA QSYSWRSTLA LSGSLCVVVS 

       190        200        210        220        230        240 
FFCLLLIHNE PADVGLRNLD PRPSKGKKGS SKEESTLQDL LLSPYLWVLS TGYLVVFGVK 

       250        260        270        280        290        300 
TCCTDWGQFF LIQERGQSAL VGSSYISALE VGGLVGSIAA GYLSDRAMAK AGLSVYGNPR 

       310        320        330        340        350        360 
HSLLLLMMAG MAASMFLFRV TVTSDSPKIW ILVLGAVFGF SSYGPIALFG VIANESAPPN 

       370        380        390        400        410        420 
LCGTSHAIVG LMANVGGFLA GLPFSTIAKH YSWSTAFWVA EVICIASTVV FFLLRNIRTK 


MGRVSKKAE 

References and documentation are available.

Number of amino acids: 429

Molecular weight: 46305.02

Theoretical pI: 9.15

Amino acid composition: 
Ala (A)  40	  9.3%
Arg (R)  14	  3.3%
Asn (N)  11	  2.6%
Asp (D)  11	  2.6%
Cys (C)   7	  1.6%
Gln (Q)  10	  2.3%
Glu (E)  10	  2.3%
Gly (G)  41	  9.6%
His (H)   4	  0.9%
Ile (I)  20	  4.7%
Leu (L)  56	 13.1%
Lys (K)  16	  3.7%
Met (M)  12	  2.8%
Phe (F)  27	  6.3%
Pro (P)  16	  3.7%
Ser (S)  51	 11.9%
Thr (T)  20	  4.7%
Trp (W)  13	  3.0%
Tyr (Y)  15	  3.5%
Val (V)  35	  8.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 21
Total number of positively charged residues (Arg + Lys): 30

Atomic composition:

Carbon      C	      2140
Hydrogen    H	      3293
Nitrogen    N	       529
Oxygen      O	       579
Sulfur      S	        19

Formula: C2140H3293N529O579S19
Total number of atoms: 6560

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    94225
Abs 0.1% (=1 g/l)   2.035, assuming all pairs of Cys residues form cystines


Ext. coefficient    93850
Abs 0.1% (=1 g/l)   2.027, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.64
This classifies the protein as stable.



Aliphatic index: 102.07

Grand average of hydropathicity (GRAVY): 0.523