ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAASGDPGSA ESYRSPLAAR YASREMCFLF SDRYKFQTWR QLWLWLAEAE QTLGLPITDE 

        70         80         90        100        110        120 
QIQEMKSNLN NIDFQMAAEE EKRLRHDVMA HVHTFGHCCP KAAGIIHLGA TSCYVGDNTD 

       130        140        150        160        170        180 
LIILRNAFDL LLPKLARVIS RLADFAKDRA DLPTLGFTHF QPAQLTTVGK RCCLWIQDSV 

       190        200        210        220        230        240 
MDLQNLKRVR DDVRFRGVKG TTGTQASFLQ LFEGDHQKVE QLDKMVTEKA GFKRAFIITG 

       250        260        270        280        290        300 
QTYTRKVDIE VLSVLASLGA SVHKICTDIR LLANLKEMEE PFEKQQIGSS AMPYKRNPMR 

       310        320        330        340        350        360 
SERCCSLARH LMALTMDPLQ TASVQWFERT LDDSANRRIC LAEAFLTADT ILNTLQNISE 

       370        380        390        400        410        420 
GLVVYPKVIE RRIRQELPFM ATENIIMAMV KAGGSRQDCH EKIRVLSQQA AAVVKQEGGD 

       430        440        450        460        470        480 
NDLIERIRAD AYFSPIHSQL EHLLDPSSFT GRAPQQVHRF LEEEVRPLLK PYGNEMAVKA 


ELCL 

References and documentation are available.

Number of amino acids: 484

Molecular weight: 54808.07

Theoretical pI: 6.90

Amino acid composition: 
Ala (A)  45	  9.3%
Arg (R)  36	  7.4%
Asn (N)  14	  2.9%
Asp (D)  28	  5.8%
Cys (C)  12	  2.5%
Gln (Q)  28	  5.8%
Glu (E)  33	  6.8%
Gly (G)  24	  5.0%
His (H)  13	  2.7%
Ile (I)  26	  5.4%
Leu (L)  53	 11.0%
Lys (K)  24	  5.0%
Met (M)  16	  3.3%
Phe (F)  21	  4.3%
Pro (P)  18	  3.7%
Ser (S)  27	  5.6%
Thr (T)  26	  5.4%
Trp (W)   5	  1.0%
Tyr (Y)   9	  1.9%
Val (V)  26	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 61
Total number of positively charged residues (Arg + Lys): 60

Atomic composition:

Carbon      C	      2414
Hydrogen    H	      3859
Nitrogen    N	       689
Oxygen      O	       711
Sulfur      S	        28

Formula: C2414H3859N689O711S28
Total number of atoms: 7701

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    41660
Abs 0.1% (=1 g/l)   0.760, assuming all pairs of Cys residues form cystines


Ext. coefficient    40910
Abs 0.1% (=1 g/l)   0.746, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 52.52
This classifies the protein as unstable.



Aliphatic index: 88.53

Grand average of hydropathicity (GRAVY): -0.257