ProtParam
User-provided sequence:
10 20 30 40 50 60
MAASIPETQK GVIFYENGGE LQYKDIPVPK PKANELLINV KYSGVCHTDL HAWKGDWPLP
70 80 90 100 110 120
TKLPLVGGHE GAGVVVAMGE NVKGWKIGDF AGIKWLNGSC MSCEYCELSN ESNCPEADLS
130 140 150 160 170 180
GYTHDGSFQQ YATADAVQAA KIPVGTDLAE VAPVLCAGVT VYKALKSANL KAGDWVAISG
190 200 210 220 230 240
AAGGLGSLAV QYAKAMGYRV LGIDAGEEKA KLFKDLGGEY FIDFTKSKNI PEEVIEATKG
250 260 270 280 290 300
GAHGVINVSV SEFAIEQSTN YVRSNGTVVL VGLPRDAKCK SDVFNQVVKS ISIVGSYVGN
310 320 330 340 350
RADTREAIDF FSRGLVKAPI HVVGLSELPS IYEKMEKGAI VGRYVVDTSK
References and
documentation are available.
Number of amino acids: 350
Molecular weight: 37260.52
Theoretical pI: 5.77
Total number of negatively charged residues (Asp + Glu): 40
Total number of positively charged residues (Arg + Lys): 35
Atomic composition:
Carbon C 1668
Hydrogen H 2622
Nitrogen N 438
Oxygen O 504
Sulfur S 12
Formula: C
1668H
2622N
438O
504S
12
Total number of atoms: 5244
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 48735
Abs 0.1% (=1 g/l) 1.308, assuming all pairs of Cys residues form cystines
Ext. coefficient 48360
Abs 0.1% (=1 g/l) 1.298, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 22.84
This classifies the protein as stable.
Aliphatic index: 89.69
Grand average of hydropathicity (GRAVY): -0.032