ProtParam
User-provided sequence:
10 20 30 40 50 60
MAASPLVVQK TEEEWRAVLS PEQFRILRQK GTEKPGTGEY DKFFEEGIFD CVGCKTPLYK
70 80 90 100 110 120
STTKFDSGCG WPAFFEGLPG AINRTPDPDG RRTEITCAAC DGHLGHVFKG EGYGNPTDER
130
HCVNSVSISF NPAKSSSII
References and
documentation are available.
Number of amino acids: 139
Molecular weight: 15251.11
Theoretical pI: 5.62
Total number of negatively charged residues (Asp + Glu): 19
Total number of positively charged residues (Arg + Lys): 16
Atomic composition:
Carbon C 673
Hydrogen H 1030
Nitrogen N 184
Oxygen O 208
Sulfur S 7
Formula: C
673H
1030N
184O
208S
7
Total number of atoms: 2102
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 15845
Abs 0.1% (=1 g/l) 1.039, assuming all pairs of Cys residues form cystines
Ext. coefficient 15470
Abs 0.1% (=1 g/l) 1.014, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 45.00
This classifies the protein as unstable.
Aliphatic index: 56.83
Grand average of hydropathicity (GRAVY): -0.501