ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAASRLCSAA AIAAAFTSMS MSQNRAYADS RFRFPFFSSS PPAEESPTDH KSSSNSKSET 

        70         80         90        100        110        120 
KPDSDEPKGS GFDPESLERG AKALREINSS PHSKQVFDLM RKQEKTRLAE LAAEKEHNEA 

       130        140        150        160        170        180 
IQASKDIERQ RKLAEDQRNL VQQQAQAKAQ NLRYEDELAR KRMQTDNEAQ RRHNAELVSM 

       190        200        210        220        230        240 
QEASSIRKEK ARIATEEQIQ AQQRETEKER AELERETIRV KAMAEAEGRA HEAKLTEEQN 

       250        260        270        280        290        300 
RRMLLDKING EREKWLAAIN TTFSHIEGGV RTLLTDRSKL IMTVGGVTAL AAGVYTTREG 

       310        320        330        340        350        360 
ARVTWGYINR ILGQPSLIRE SSMGRFPWAG SVSQFKNKLS TAAGAAASAE GEKPLENVIL 

       370        380        390        400        410        420 
HRSLKTRIER LARATANTKS HKAPFRNMMF YGPPGTGKTM VAREIARKSG LDYAMMTGGD 

       430        440        450        460        470        480 
VAPLGAQAVT KIHEIFDWAK KSNKGLLLFI DEADAFLCER NSTYMSEAQR SALNALLFRT 

       490        500        510        520        530        540 
GDQSRDIVLV LATNRPGDLD SAVTDRIDEV IEFPLPGEEE RFKLLKLYLN KYLMGDDKKG 

       550        560        570        580        590        600 
EKDSNLKWSN LFKKKKSQKI TIEGDLTDQV IKEAAKKTEG FSGREIAKLV AGVQAAVYGR 

       610        620        630 
QDCVLDSQLF EEIVDYKIEE HHQRIRLATE GGQSFP 

References and documentation are available.

Number of amino acids: 636

Molecular weight: 71194.47

Theoretical pI: 8.99

Amino acid composition: 
Ala (A)  73	 11.5%
Arg (R)  51	  8.0%
Asn (N)  23	  3.6%
Asp (D)  31	  4.9%
Cys (C)   3	  0.5%
Gln (Q)  31	  4.9%
Glu (E)  62	  9.7%
Gly (G)  37	  5.8%
His (H)  11	  1.7%
Ile (I)  31	  4.9%
Leu (L)  52	  8.2%
Lys (K)  50	  7.9%
Met (M)  17	  2.7%
Phe (F)  22	  3.5%
Pro (P)  19	  3.0%
Ser (S)  49	  7.7%
Thr (T)  34	  5.3%
Trp (W)   5	  0.8%
Tyr (Y)  11	  1.7%
Val (V)  24	  3.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 93
Total number of positively charged residues (Arg + Lys): 101

Atomic composition:

Carbon      C	      3088
Hydrogen    H	      5002
Nitrogen    N	       920
Oxygen      O	       971
Sulfur      S	        20

Formula: C3088H5002N920O971S20
Total number of atoms: 10001

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    44015
Abs 0.1% (=1 g/l)   0.618, assuming all pairs of Cys residues form cystines


Ext. coefficient    43890
Abs 0.1% (=1 g/l)   0.616, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.78
This classifies the protein as unstable.



Aliphatic index: 73.32

Grand average of hydropathicity (GRAVY): -0.677