ProtParam
User-provided sequence:
10 20 30 40 50 60
MAATAATTTS SHLLLSSSRH VASSSQPSIL SPRSLFSNNG KRAPTGVRNH QYASGVRCMA
70 80 90 100 110 120
VAADAAETKT AARKKSGYEL QTLTGWLLRQ EMKGEIDAEL TIVMSSISLA CKQIASLVQR
130 140 150 160 170 180
AGISNLTGVQ GAVNIQGEDQ KKLDVISNEV FSNCLRSSGR TGIIASEEED VPVAVEESYS
190 200 210 220 230 240
GNYVVVFDPL DGSSNIDAAV STGSIFGIYS PNDECIVDDS DDISALGSEE QRCIVNVCQP
250 260 270 280 290 300
GNNLLAAGYC MYSSSVIFVL TLGKGVFSFT LDPMYGEFVL TQENIEIPKA GRIYSFNEGN
310 320 330 340 350 360
YQMWDDKLKK YIDDLKDPGP TGKPYSARYI GSLVGDFHRT LLYGGIYGYP RDAKSKNGKL
370 380 390 400 410
RLLYECAPMS FIVEQAGGKG SDGHSRVLDI QPTEIHQRVP LYIGSTEEVE KLEKYLA
References and
documentation are available.
Number of amino acids: 417
Molecular weight: 45161.89
Theoretical pI: 5.25
Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 39
Atomic composition:
Carbon C 1981
Hydrogen H 3137
Nitrogen N 539
Oxygen O 634
Sulfur S 16
Formula: C
1981H
3137N
539O
634S
16
Total number of atoms: 6307
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 39810
Abs 0.1% (=1 g/l) 0.881, assuming all pairs of Cys residues form cystines
Ext. coefficient 39310
Abs 0.1% (=1 g/l) 0.870, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 43.98
This classifies the protein as unstable.
Aliphatic index: 85.83
Grand average of hydropathicity (GRAVY): -0.208