ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAATATEKLP QLKSAVDGLT EMSENEKSGF INLVSRYLSG EAQHIEWSKI QTPTDEIVVP 

        70         80         90        100        110        120 
YDKMANVSED ASETKYLLDK LVVLKLNGGL GTTMGCTGPK SVIEVRDGLT FLDLIVIQIE 

       130        140        150        160        170        180 
NLNNKYNCKV PLVLMNSFNT HDDTQKIVEK YTKSNVDIHT FNQSKYPRVV ADEFVPWPSK 

       190        200        210        220        230        240 
GKTDKDGWYP PGHGDVFPSL MNSGKLDAFL SQGKEYVFIA NSDNLGAIVD LKILKHLIQN 

       250        260        270        280        290        300 
KNEYCMEVTP KTLADVKGGT LISYEGKVQL LEIAQVPDEH VNEFKSIEKF KIFNTNNLWV 

       310        320        330        340        350        360 
NLKAIKKLVE ADALKMEIIP NPKEVDGVKV LQLETAAGAA IRFFDNAIGV NVPRSRFLPV 

       370        380        390        400        410        420 
KATSDLLLVQ SDLYTLVDGF VTRNKARTNP TNPAIELGPE FKKVASFLSR FKSIPSIVEL 

       430        440        450        460        470 
DSLKVSGDVW FGSGVVLKGK VTVKANAGTK LEIPDNAVLE NKDINGPEDL 

References and documentation are available.

Number of amino acids: 470

Molecular weight: 51919.64

Theoretical pI: 5.72

Amino acid composition: 
Ala (A)  28	  6.0%
Arg (R)   9	  1.9%
Asn (N)  32	  6.8%
Asp (D)  30	  6.4%
Cys (C)   3	  0.6%
Gln (Q)  12	  2.6%
Glu (E)  31	  6.6%
Gly (G)  30	  6.4%
His (H)   6	  1.3%
Ile (I)  28	  6.0%
Leu (L)  48	 10.2%
Lys (K)  46	  9.8%
Met (M)   8	  1.7%
Phe (F)  19	  4.0%
Pro (P)  23	  4.9%
Ser (S)  29	  6.2%
Thr (T)  27	  5.7%
Trp (W)   5	  1.1%
Tyr (Y)  11	  2.3%
Val (V)  45	  9.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 61
Total number of positively charged residues (Arg + Lys): 55

Atomic composition:

Carbon      C	      2338
Hydrogen    H	      3732
Nitrogen    N	       604
Oxygen      O	       704
Sulfur      S	        11

Formula: C2338H3732N604O704S11
Total number of atoms: 7389

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    44015
Abs 0.1% (=1 g/l)   0.848, assuming all pairs of Cys residues form cystines


Ext. coefficient    43890
Abs 0.1% (=1 g/l)   0.845, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 24.35
This classifies the protein as stable.



Aliphatic index: 96.79

Grand average of hydropathicity (GRAVY): -0.197