ProtParam
User-provided sequence:
10 20 30 40 50 60
MAATLPLSPI NHQLCRFGNN SLTTHRFCSP GFLISSPCFI GLTGMGSATQ LRARRSLISS
70 80 90 100 110 120
AVATNSLLHD VGATVAVLGG AYALVLSFES LTKRNVIQQS LSRKLVHILS GLLFVLAWPI
130 140 150 160 170 180
FSGSTEARYF AAFVPLVNGL RLVINGLSIS PNSMLIKSVT REGRAEELLK GPLFYVLALL
190 200 210 220 230 240
FSAVFFWRES PIGMISLAMM CGGDGIADIM GRKFGSTKIP YNPRKSWAGS ISMFIFGFFI
250 260 270 280 290 300
SIALLYYYSS LGYLHMNWET TLQRVAMVSM VATVVESLPI TDQLDDNISV PLATILAAYL
SFGY
References and
documentation are available.
Number of amino acids: 304
Molecular weight: 33089.86
Theoretical pI: 9.48
Total number of negatively charged residues (Asp + Glu): 14
Total number of positively charged residues (Arg + Lys): 22
Atomic composition:
Carbon C 1516
Hydrogen H 2385
Nitrogen N 387
Oxygen O 411
Sulfur S 15
Formula: C
1516H
2385N
387O
411S
15
Total number of atoms: 4714
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 37150
Abs 0.1% (=1 g/l) 1.123, assuming all pairs of Cys residues form cystines
Ext. coefficient 36900
Abs 0.1% (=1 g/l) 1.115, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 35.35
This classifies the protein as stable.
Aliphatic index: 109.74
Grand average of hydropathicity (GRAVY): 0.553