ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAATSNSGED PTLSYHHRSP FRFELLQSIS SSDPRYSSLT PSSTNRPFSV SQSLPNSQLS 

        70         80         90        100        110        120 
PLISPHWDDS YSQITQKVQK SRKNHRIQLG SIANMSGGES IDIAKVIVKQ ESSPQNVKRV 

       130        140        150        160        170        180 
YNKSKGGTKL LKAGKRMANG EVQNGGLNGA SINCRYDSSL GLLTKKFVKL IQEAEDGTLD 

       190        200        210        220        230        240 
LNYCAVVLEV QKRRIYDITN VLEGIGLIEK TTKNHIRWKG ADNLGQKDLG DQISRLKSEV 

       250        260        270        280        290        300 
ESMQSEESRL DDLIRERQEA LRSLEEDDYC RRYMFMTEED ITSLPRFQNQ TLLAIKAPTA 

       310        320        330        340        350        360 
SYIEVPDPDE MSFPQQYRMV IRSRMGPIDV YLLSKYKGDS AETSDKLGNE SDQKAPVGVD 

       370        380        390 
TPSLKIVTSD TDLKADYWFE SDAEVSLTDL WSNFNS 

References and documentation are available.

Number of amino acids: 396

Molecular weight: 44487.85

Theoretical pI: 5.74

Amino acid composition: 
Ala (A)  18	  4.5%
Arg (R)  23	  5.8%
Asn (N)  19	  4.8%
Asp (D)  29	  7.3%
Cys (C)   3	  0.8%
Gln (Q)  21	  5.3%
Glu (E)  26	  6.6%
Gly (G)  22	  5.6%
His (H)   5	  1.3%
Ile (I)  23	  5.8%
Leu (L)  37	  9.3%
Lys (K)  27	  6.8%
Met (M)   9	  2.3%
Phe (F)   9	  2.3%
Pro (P)  17	  4.3%
Ser (S)  50	 12.6%
Thr (T)  20	  5.1%
Trp (W)   4	  1.0%
Tyr (Y)  14	  3.5%
Val (V)  20	  5.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 55
Total number of positively charged residues (Arg + Lys): 50

Atomic composition:

Carbon      C	      1935
Hydrogen    H	      3094
Nitrogen    N	       546
Oxygen      O	       631
Sulfur      S	        12

Formula: C1935H3094N546O631S12
Total number of atoms: 6218

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    42985
Abs 0.1% (=1 g/l)   0.966, assuming all pairs of Cys residues form cystines


Ext. coefficient    42860
Abs 0.1% (=1 g/l)   0.963, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 52.93
This classifies the protein as unstable.



Aliphatic index: 78.28

Grand average of hydropathicity (GRAVY): -0.654