ProtParam
User-provided sequence:
10 20 30 40 50 60
MAATTENLPQ LKSAVDGLTE MSESEKSGFI SLVSRYLSGE AQHIEWSKIQ TPTDEIVVPY
70 80 90 100 110 120
EKMTPVSQDV AETKNLLDKL VVLKLNGGLG TTMGCTGPKS VIEVRDGLTF LDLIVIQIEN
130 140 150 160 170 180
LNNKYGCKVP LVLMNSFNTH DDTHKIVEKY TNSNVDIHTF NQSKYPRVVA DEFVPWPSKG
190 200 210 220 230 240
KTDKEGWYPP GHGDVFPALM NSGKLDTFLS QGKEYVFVAN SDNLGAIVDL TILKHLIQNK
250 260 270 280 290 300
NEYCMEVTPK TLADVKGGTL ISYEGKVQLL EIAQVPDEHV NEFKSIEKFK IFNTNNLWVN
310 320 330 340 350 360
LKAIKKLVEA DALKMEIIPN PKEVDGVKVL QLETAAGAAI RFFDNAIGVN VPRSRFLPVK
370 380 390 400 410 420
ASSDLLLVQS DLYTLVDGFV TRNKARTNPS NPSIELGPEF KKVATFLSRF KSIPSIVELD
430 440 450 460
SLKVSGDVWF GSSIVLKGKV TVAAKSGVKL EIPDRAVVEN KNINGPEDL
References and
documentation are available.
Number of amino acids: 469
Molecular weight: 51738.44
Theoretical pI: 5.80
Total number of negatively charged residues (Asp + Glu): 59
Total number of positively charged residues (Arg + Lys): 53
Atomic composition:
Carbon C 2329
Hydrogen H 3721
Nitrogen N 603
Oxygen O 701
Sulfur S 11
Formula: C
2329H
3721N
603O
701S
11
Total number of atoms: 7365
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 42525
Abs 0.1% (=1 g/l) 0.822, assuming all pairs of Cys residues form cystines
Ext. coefficient 42400
Abs 0.1% (=1 g/l) 0.820, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 27.69
This classifies the protein as stable.
Aliphatic index: 97.38
Grand average of hydropathicity (GRAVY): -0.161