ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAAVPTIDLT LADSDNEDIF HSFSSSTSVD KIDIRKENGK LRMAGLEVAQ SNDDAARQAF 

        70         80         90        100        110        120 
HVFKTNISNN ETFDTILSKS KTITDSTFNN EKSSNEVKQQ QVLKEETMGS SNDEKKTQES 

       130        140        150        160        170        180 
SPSAEMIKLF YENDDVPLSD SFKQKEEGKR INQDEQVKEN ICGISSSYVS KDYDGVEDDF 

       190        200        210        220        230        240 
EPNTCQDSNL DFQEEKLNLN NKPSQQQFSD PETKDNSLKS ENKDQIKGVT TTSYRDLPIE 

       250        260        270        280        290        300 
SSAFQDSETQ NNSKNTIPNI VNEKRTPALP SNLSSVESSL KNETAKVEGK TTVRLPGLQN 

       310        320        330        340        350        360 
NVALLEQEQS ELFKHFSEQP VDISDFGRKI KRKHSGDFAD NKILKRPILP SKNMDHTTHN 

       370        380        390        400        410        420 
SHDSEQKNSS IIILSDEDES GAGINDIESP LKVSEPNTAD ALRSSVPEVI SLLDLPNIDL 

       430        440        450        460        470        480 
NNSVIKEASG SNSIPTSETD AQSSSSSVLQ GTIMTEQATQ SSQHECNSSL DTLKKNHQKL 

       490        500        510        520        530        540 
LKDLNSRESE LRNALSCCKT NSEILRRKLS RREKEVSDAE KHWQLLLTSM ARGGRTISST 

       550        560        570        580        590        600 
QQILVDEAEN QLNKLKEKRQ LTKSKLDSIN LKMYNYNEQW KSFVHSKNIN LQKSLAALER 

       610        620        630        640        650        660 
SARDSKASAT VNKRNECLAE KEKLDQMLKE GTLSFSTYKQ LTGEIQQKLN DLKLGDQRTT 

       670        680        690        700        710        720 
DINSVLPIVR QPLAKRDLFI KSIDTAKDLL AKNTSRTEMT KRILYRHLDN LVSYKNFFED 

       730        740        750        760        770        780 
GRSLIDINRR HVAHESAQIL FTNGVKMPIV FETLQDYGIK FSNPAIVNPD RRAQYFKSIE 

       790        800        810        820        830        840 
VARDLISKST RSEDAKRKIT RFLNIIEEFR KDIDTGFPPT PLKREGVGKA VVGLRQQGLK 

       850        860        870        880        890        900 
MDRLYENLRR YKIPITSEEL LQQSYLFPVN ADQRPPSNWN IVENTEDTSS TANDLSMQDE 

       910        920        930        940        950        960 
FHISNMHAAE DQEQIRALLE NVKQSESIID GEALTPEDMT VNLLKHQRLG LHWLLQVENS 

       970        980        990       1000       1010       1020 
AKKGGLLADD MGLGKTIQAI ALMLANRSEE SKCKTNLIVA PVSVLRVWKG ELETKVKKRA 

      1030       1040       1050       1060       1070       1080 
KFTTFIFGGS GNGKVKHWRD LARYDAVLVS YQTLANEFKK HWPKKLDGEQ NQLPAVPHIQ 

      1090       1100       1110       1120       1130       1140 
ALNRLKTSNE YYSPFFCNDS TFYRILLDEG QNIKNKNTRA SKACCTINGM YRWVLSGTPI 

      1150       1160       1170       1180       1190       1200 
QNSMDELYSL IRFLRIPPYH KEQRFKLDIG RFFQRNKQYQ YDNEDRKNAL RKVRVLLNAI 

      1210       1220       1230       1240       1250       1260 
MLRRSKADKI DGKPLLELPP KIVEVDESRL KGEELKFYTA LESKNQALAK KLLNNSTRGS 

      1270       1280       1290       1300       1310       1320 
YSSVLTLLLR LRQACCHSEL VVMGEKKAEG TKVANGKSFE DDWLRLYYKI THMSGEAQAQ 

      1330       1340       1350       1360       1370       1380 
VITSMNSMTC FWCMEQLEPE AMSVLTGCGH LICDTCIEPF IEESSMLPQA KKTKGGAFAI 

      1390       1400       1410       1420       1430       1440 
PCKDCQRLTN EKDIVSHKLY DQVINQGFTE EDLHAEYLSE MEKQKIQQKN VYVPNFESLE 

      1450       1460       1470       1480       1490       1500 
PSTKIEQCIQ VIQRVFDESA TEKIIIFSQF TTFFEILEHF LKNKLNFPYL KYIGSMNAQR 

      1510       1520       1530       1540       1550       1560 
RSDVINEFYR DPEKRILLIS MKAGNSGLTL TCANHVVIVD PFWNPYVEEQ AQDRCYRISQ 

      1570       1580       1590       1600       1610 
TKKVQVHKLF IKDSVEDRIS ELQKRKKEMV DSAMDPGKIK EVNSLGRREL GFLFGLNAL 

References and documentation are available.

Number of amino acids: 1619

Molecular weight: 184405.70

Theoretical pI: 7.33

Amino acid composition: 
Ala (A)  83	  5.1%
Arg (R)  83	  5.1%
Asn (N) 108	  6.7%
Asp (D)  99	  6.1%
Cys (C)  22	  1.4%
Gln (Q)  88	  5.4%
Glu (E) 130	  8.0%
Gly (G)  63	  3.9%
His (H)  30	  1.9%
Ile (I)  99	  6.1%
Leu (L) 158	  9.8%
Lys (K) 146	  9.0%
Met (M)  32	  2.0%
Phe (F)  60	  3.7%
Pro (P)  54	  3.3%
Ser (S) 151	  9.3%
Thr (T)  88	  5.4%
Trp (W)  11	  0.7%
Tyr (Y)  36	  2.2%
Val (V)  78	  4.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 229
Total number of positively charged residues (Arg + Lys): 229

Atomic composition:

Carbon      C	      8065
Hydrogen    H	     12971
Nitrogen    N	      2281
Oxygen      O	      2549
Sulfur      S	        54

Formula: C8065H12971N2281O2549S54
Total number of atoms: 25920

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   115515
Abs 0.1% (=1 g/l)   0.626, assuming all pairs of Cys residues form cystines


Ext. coefficient   114140
Abs 0.1% (=1 g/l)   0.619, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 49.71
This classifies the protein as unstable.



Aliphatic index: 81.01

Grand average of hydropathicity (GRAVY): -0.661