ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAAVRDYKTA LEFAKSLPRL DGLSVQELMD SKTRGGLTYN DFLVLPGLVD FPSSEVSLQT 

        70         80         90        100        110        120 
KLTRNITLNT PFVSSPMDTV TESEMAIFMA LLGGIGFIHH NCTPEDQADM VRRVKNYENG 

       130        140        150        160        170        180 
FINNPIVISP TTTVGEAKSM KERFGFSGFP VTEDGKRNGK LMGIVTSRDI QFVEDNSLLV 

       190        200        210        220        230        240 
QDVMTKNPVT GAQGITLSEG NEILKKIKKG KLLIVDDNGN LVSMLSRTDL MKNQNYPLAS 

       250        260        270        280        290        300 
KSATTKQLLC GAAIGTIDAD KERLRLLVEA GLDVVILDSS QGNSIFQLNM IKWIKETFPD 

       310        320        330        340        350        360 
LEIIAGNVAT REQAANLIAA GADGLRIGMG SGSICITQEV MACGRPQGTA VYNVCEFANQ 

       370        380        390        400        410        420 
FGIPCMADGG VQNIGHITKA LALGSSTVMM GGMLAGTTES PGEYFYQDGK RLKAYRGMGS 

       430        440        450        460        470        480 
IDAMQKTGTK GNASTSRYFS ESDSVLVAQG VSGAVVDKGS IKKFIPYLYN GLQHSCQDIG 

       490        500        510        520 
YKSLTLLKEN VQSGKVRFEF RTASAQLEGG VHNLHSYEKR LHN 

References and documentation are available.

Number of amino acids: 523

Molecular weight: 56584.78

Theoretical pI: 7.04

Amino acid composition: 
Ala (A)  36	  6.9%
Arg (R)  21	  4.0%
Asn (N)  28	  5.4%
Asp (D)  27	  5.2%
Cys (C)   7	  1.3%
Gln (Q)  22	  4.2%
Glu (E)  27	  5.2%
Gly (G)  53	 10.1%
His (H)   7	  1.3%
Ile (I)  32	  6.1%
Leu (L)  49	  9.4%
Lys (K)  33	  6.3%
Met (M)  20	  3.8%
Phe (F)  20	  3.8%
Pro (P)  16	  3.1%
Ser (S)  40	  7.6%
Thr (T)  35	  6.7%
Trp (W)   1	  0.2%
Tyr (Y)  13	  2.5%
Val (V)  36	  6.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 54
Total number of positively charged residues (Arg + Lys): 54

Atomic composition:

Carbon      C	      2480
Hydrogen    H	      4000
Nitrogen    N	       684
Oxygen      O	       770
Sulfur      S	        27

Formula: C2480H4000N684O770S27
Total number of atoms: 7961

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    25245
Abs 0.1% (=1 g/l)   0.446, assuming all pairs of Cys residues form cystines


Ext. coefficient    24870
Abs 0.1% (=1 g/l)   0.440, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 31.34
This classifies the protein as stable.



Aliphatic index: 87.25

Grand average of hydropathicity (GRAVY): -0.140