ProtParam
User-provided sequence:
10 20 30 40 50 60
MAAVTLHLRA ETKPLEARAA LTPTTVKKLI AKGFKIYVED SPQSTFNINE YRQAGAIIVP
70 80 90 100 110 120
AGSWKTAPRD RIIIGLKEMP ETDTFPLVHE HIQFAHCYKD QAGWQNVLMR FIKGHGTLYD
130 140 150 160 170 180
LEFLENDQGR RVAAFGFYAG FAGAALGVRD WAFKQTHSDD EDLPAVSPYP NEKALVKDVT
190 200 210 220 230 240
KDYKEALATG ARKPTVLIIG ALGRCGSGAI DLLHKVGIPD ANILKWDIKE TSRGGPFDEI
250 260 270 280 290 300
PQADIFINCI YLSKPIAPFT NMEKLNNPNR RLRTVVDVSA DTTNPHNPIP IYTVATVFNK
310 320 330 340 350 360
PTVLVPTTAG PKLSVISIDH LPSLLPREAS EFFSHDLLPS LELLPQRKTA PVWVRAKKLF
370
DRHCARVKRS SRL
References and
documentation are available.
Number of amino acids: 373
Molecular weight: 41464.83
Theoretical pI: 9.18
Total number of negatively charged residues (Asp + Glu): 40
Total number of positively charged residues (Arg + Lys): 48
Atomic composition:
Carbon C 1878
Hydrogen H 2967
Nitrogen N 515
Oxygen O 528
Sulfur S 8
Formula: C
1878H
2967N
515O
528S
8
Total number of atoms: 5896
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 41160
Abs 0.1% (=1 g/l) 0.993, assuming all pairs of Cys residues form cystines
Ext. coefficient 40910
Abs 0.1% (=1 g/l) 0.987, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 34.71
This classifies the protein as stable.
Aliphatic index: 91.31
Grand average of hydropathicity (GRAVY): -0.210