ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MACENNLVVK QIMDLYNQIS NLESLKPSKN VDTLFRQLVS TCLPTDTNID VTEIHDEKVK 

        70         80         90        100        110        120 
DMRSHLIKLC GEAEGYLEQH FSAILGSFED NPLNHLHIFP YYNNYLKLGK LEFDLLSQHT 

       130        140        150        160        170        180 
THVPTKVAFI GSGPMPLTSI VLAKFHLPNT TFHNFDIDSH ANTLASNLVS RDSDLSKRMI 

       190        200        210        220        230        240 
FHTTDVLNAK EGLDQYDVVF LAALVGMDKE SKVKAIEHLE KHMAPGAVVM LRSAHGLRAF 

       250        260        270        280        290        300 
LYPIVDSCDL KGFEVLTIYH PSDDVVNSVV IARKLGGSNG ARGSQIGRCV VMPCNCSKVH 

       310        320 
AILNNRGMEK NLIEEYSAIE 

References and documentation are available.

Number of amino acids: 320

Molecular weight: 35678.96

Theoretical pI: 6.04

Amino acid composition: 
Ala (A)  19	  5.9%
Arg (R)  10	  3.1%
Asn (N)  21	  6.6%
Asp (D)  20	  6.2%
Cys (C)   7	  2.2%
Gln (Q)   7	  2.2%
Glu (E)  18	  5.6%
Gly (G)  17	  5.3%
His (H)  16	  5.0%
Ile (I)  19	  5.9%
Leu (L)  37	 11.6%
Lys (K)  20	  6.2%
Met (M)  10	  3.1%
Phe (F)  13	  4.1%
Pro (P)  12	  3.8%
Ser (S)  24	  7.5%
Thr (T)  15	  4.7%
Trp (W)   0	  0.0%
Tyr (Y)   9	  2.8%
Val (V)  26	  8.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 38
Total number of positively charged residues (Arg + Lys): 30

Atomic composition:

Carbon      C	      1583
Hydrogen    H	      2510
Nitrogen    N	       430
Oxygen      O	       473
Sulfur      S	        17

Formula: C1583H2510N430O473S17
Total number of atoms: 5013

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    13785
Abs 0.1% (=1 g/l)   0.386, assuming all pairs of Cys residues form cystines


Ext. coefficient    13410
Abs 0.1% (=1 g/l)   0.376, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.25
This classifies the protein as stable.



Aliphatic index: 97.75

Grand average of hydropathicity (GRAVY): -0.094