ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MACNFQFPEI AYPGKLICPQ YGTENKDGED IIFNYVPGPG TKLIQYEHNG RTLEAITATL 

        70         80         90        100        110        120 
VGTVRCEEEK KTDQEEEREG TDQSTEEEKS VDASPNDVTR RTVKNILVSV LPGTEKGRKT 

       130        140        150        160        170        180 
NKYANNDFAN NLPKEGDIVL TRVTRLSLQR ANVEILAVED KPSPIDSGIG SNGSGIVAAG 

       190        200        210        220        230        240 
GGSGAATFSV SQASSDLGET FRGIIRSQDV RSTDRDRVKV IECFKPGDIV RAQVLSLGDG 

       250        260        270        280        290 
TNYYLTTARN DLGVVFARAA NGAGGLMYAT DWQMMTSPVT GATEKRKCAK PF 

References and documentation are available.

Number of amino acids: 292

Molecular weight: 31583.32

Theoretical pI: 5.24

Amino acid composition: 
Ala (A)  23	  7.9%
Arg (R)  18	  6.2%
Asn (N)  16	  5.5%
Asp (D)  18	  6.2%
Cys (C)   5	  1.7%
Gln (Q)  10	  3.4%
Glu (E)  22	  7.5%
Gly (G)  29	  9.9%
His (H)   1	  0.3%
Ile (I)  16	  5.5%
Leu (L)  17	  5.8%
Lys (K)  17	  5.8%
Met (M)   4	  1.4%
Phe (F)   9	  3.1%
Pro (P)  13	  4.5%
Ser (S)  18	  6.2%
Thr (T)  25	  8.6%
Trp (W)   1	  0.3%
Tyr (Y)   8	  2.7%
Val (V)  22	  7.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 40
Total number of positively charged residues (Arg + Lys): 35

Atomic composition:

Carbon      C	      1365
Hydrogen    H	      2192
Nitrogen    N	       392
Oxygen      O	       450
Sulfur      S	         9

Formula: C1365H2192N392O450S9
Total number of atoms: 4408

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    17670
Abs 0.1% (=1 g/l)   0.559, assuming all pairs of Cys residues form cystines


Ext. coefficient    17420
Abs 0.1% (=1 g/l)   0.552, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.39
This classifies the protein as unstable.



Aliphatic index: 73.80

Grand average of hydropathicity (GRAVY): -0.484