ProtParam
User-provided sequence:
10 20 30 40 50 60
MADDKEMPAA VVDGHDQVTG HIISTTIGGK NGEPKQTISY MAERVVGTGS FGIVFQAKCL
70 80 90 100 110 120
ETGETVAIKK VLQDRRYKNR ELQLMRVMDH PNVVCLKHCF FSTTSKDELF LNLVMEYVPE
130 140 150 160 170 180
SLYRVLKHYS SANQRMPLVY VKLYMYQIFR GLAYIHNVAG VCHRDLKPQN LLVDPLTHQV
190 200 210 220 230 240
KICDFGSAKQ LVKGEANISY ICSRFYRAPE LIFGATEYTT SIDIWSAGCV LAELLLGQPL
250 260 270 280 290 300
FPGENAVDQL VEIIKVLGTP TREEIRCMNP HYTDFRFPQI KAHPWHKIFH KRMPPEAIDF
310 320 330 340 350 360
ASRLLQYSPS LRCTALEACA HPFFDELREP NARLPNGRPF PPLFNFKQEV AGSSPELVNK
370 380
LIPDHIKRQL GLSFLNQSGT
References and
documentation are available.
Number of amino acids: 380
Molecular weight: 43098.90
Theoretical pI: 8.43
Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 43
Atomic composition:
Carbon C 1942
Hydrogen H 3039
Nitrogen N 527
Oxygen O 543
Sulfur S 20
Formula: C
1942H
3039N
527O
543S
20
Total number of atoms: 6071
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 32485
Abs 0.1% (=1 g/l) 0.754, assuming all pairs of Cys residues form cystines
Ext. coefficient 31860
Abs 0.1% (=1 g/l) 0.739, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 47.06
This classifies the protein as unstable.
Aliphatic index: 88.50
Grand average of hydropathicity (GRAVY): -0.187