ProtParam
User-provided sequence:
10 20 30 40 50 60
MADEKRLEDL RSKIMESIGK SEKDVVPIEN KRFNTDNAVI DTHFKRQKSD GELPKAPKSR
70 80 90 100 110 120
NVSHSNNRGP SSIITMSTNR TTYEQTRAGP HRQSYRDASG RSYNRENRYS SHNTGPQWNN
130 140 150 160 170 180
NPYNRQRDER RGRNERFDRR GRNGNGNYDR FNYQRKNEGS KFNGDRDKRQ LQTNKYDMNY
190 200 210 220 230 240
NSQNVMYPGS SFDSPAYYNM ASSKANSRLV ISGLSQSSDP SIVARLKDLL ENFISGLQKT
250 260 270 280 290 300
ESNAEDFKIS NFYIGEGRPD HIIVEFSSQI CSTMVLACRS FFNAKLGTFD LKWRRPNDYV
310 320 330 340 350 360
QQLDHLVDFC RGTVIALENL ENIGEGEDYR MKELFSSLNV TNGTAKPLFY KCSSNTNNTG
370 380 390 400 410 420
KESEFTKCIL LSFEVVTQDI LDKLKPYKWF KPNDGKISQV TSWITFQSLP NLVTQSVRVE
430 440 450 460 470 480
SRVLLLLNCL DPLDLKDETF ITEIKETLKY SIAGADTIKI CQPGVDYRLN FENLASGAGN
490 500 510 520
IYIKFKTLEA AKHAMEELPG TQFNDRTVLC TYIDEDDFDM MEATQLS
References and
documentation are available.
Number of amino acids: 527
Molecular weight: 60459.53
Theoretical pI: 8.41
Total number of negatively charged residues (Asp + Glu): 70
Total number of positively charged residues (Arg + Lys): 74
Atomic composition:
Carbon C 2634
Hydrogen H 4132
Nitrogen N 762
Oxygen O 836
Sulfur S 19
Formula: C
2634H
4132N
762O
836S
19
Total number of atoms: 8383
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 53790
Abs 0.1% (=1 g/l) 0.890, assuming all pairs of Cys residues form cystines
Ext. coefficient 53290
Abs 0.1% (=1 g/l) 0.881, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.73
This classifies the protein as stable.
Aliphatic index: 66.60
Grand average of hydropathicity (GRAVY): -0.809