ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MADESLHTVP LEHNIDYHIV TLFERLEAMR KDSHGGGHGV NNRLSSTLQA PKRSMQAEIR 

        70         80         90        100        110        120 
TLEFWRSIIS ECLASFMYVF IVCGAAAGVG VGASVSSVLL ATALASGLAM ATLTQCFLHI 

       130        140        150        160        170        180 
SGAHINPAVT LALCVVRSIS PIRAAMYITA QCGGGIAGAA LLYGVTVPGY QGNLQAAISH 

       190        200        210        220        230        240 
SAALAAWERF GVEFILTFLV VLCYFVSTDP MKKFMGNSAA SIGCAYSACC FVSMPYLNPA 

       250        260        270        280        290        300 
RSLGPSFVLN KWDSHWVYWF GPLVGGMASG LVYEYIFNSR NRNLRHNKGS IDNDSSSIHS 

       310        320        330        340        350        360 
EDELNYDMDM EKPNKYQQSQ GTYPRGQSNG NGGGQAAGNG QHQAANMGQM PGVVANAGQG 

       370        380        390        400        410        420 
NYCQNLYTAP PLSSKYDQQQ EPLYGGTRSL YCRSPTLTRS NLNRSQSVYA KSNTAINRDI 

       430        440        450        460        470        480 
VPRPGPLVPA QSLYPMRTQQ QQQQQQQQQQ QVAPAPQSSH LQNQNVQNQM QQRSESIYGM 

       490        500        510        520        530        540 
RGSMRGQQQP IQQQQQQQQQ QQLQQQQPNM GVQQQQMQPP PQMMSDPQQQ PQGFQPVYGT 

       550        560        570        580        590        600 
RTNPTPMDGN HKYDRRDPQQ MYGVTGPRNR GQSAQSDDSS YGSYHGSAVT PPARHPSVEP 

       610        620        630        640        650        660 
SPPPPPMLMY APPPQPNAAH PQPIRTQSER KVSAPVVVSQ PAACAVTYTT SQGSAVTAQQ 

       670        680        690 
QQQQQQQQQQ QQQQQQQQMM MQQQQQHYGM LPLRPN 

References and documentation are available.

Number of amino acids: 696

Molecular weight: 76508.83

Theoretical pI: 8.94

Amino acid composition: 
Ala (A)  59	  8.5%
Arg (R)  33	  4.7%
Asn (N)  35	  5.0%
Asp (D)  18	  2.6%
Cys (C)  12	  1.7%
Gln (Q) 104	 14.9%
Glu (E)  17	  2.4%
Gly (G)  55	  7.9%
His (H)  18	  2.6%
Ile (I)  23	  3.3%
Leu (L)  43	  6.2%
Lys (K)  12	  1.7%
Met (M)  30	  4.3%
Phe (F)  15	  2.2%
Pro (P)  55	  7.9%
Ser (S)  62	  8.9%
Thr (T)  29	  4.2%
Trp (W)   5	  0.7%
Tyr (Y)  30	  4.3%
Val (V)  41	  5.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 35
Total number of positively charged residues (Arg + Lys): 45

Atomic composition:

Carbon      C	      3306
Hydrogen    H	      5157
Nitrogen    N	       987
Oxygen      O	      1027
Sulfur      S	        42

Formula: C3306H5157N987O1027S42
Total number of atoms: 10519

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    72950
Abs 0.1% (=1 g/l)   0.953, assuming all pairs of Cys residues form cystines


Ext. coefficient    72200
Abs 0.1% (=1 g/l)   0.944, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 69.17
This classifies the protein as unstable.



Aliphatic index: 62.54

Grand average of hydropathicity (GRAVY): -0.591