ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MADESSDAAG EPQPAPAPVR RRSSANYRAY ATEPHAKKKS KISASRKLQL KTLMLQIAKQ 

        70         80         90        100        110        120 
EMEREAEERR GEKGRVLSTR CQPLVLDGLG FEELQDLCRQ LHARVDKVDE ERYDVEAKVT 

       130        140        150        160        170        180 
KNITEIADLT QKIYDLRGKF KRPTLRRVRI SADAMMQALL GTRAKESLDL RAHLKQVKKE 

       190        200        210 
DIEKENREVG DWRKNIDALS GMEGRKKKFE G 

References and documentation are available.

Number of amino acids: 211

Molecular weight: 24159.67

Theoretical pI: 9.57

Amino acid composition: 
Ala (A)  21	 10.0%
Arg (R)  23	 10.9%
Asn (N)   4	  1.9%
Asp (D)  14	  6.6%
Cys (C)   2	  0.9%
Gln (Q)  10	  4.7%
Glu (E)  22	 10.4%
Gly (G)  11	  5.2%
His (H)   3	  1.4%
Ile (I)   8	  3.8%
Leu (L)  20	  9.5%
Lys (K)  23	 10.9%
Met (M)   6	  2.8%
Phe (F)   3	  1.4%
Pro (P)   7	  3.3%
Ser (S)  11	  5.2%
Thr (T)   8	  3.8%
Trp (W)   1	  0.5%
Tyr (Y)   4	  1.9%
Val (V)  10	  4.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 46

Atomic composition:

Carbon      C	      1039
Hydrogen    H	      1736
Nitrogen    N	       324
Oxygen      O	       321
Sulfur      S	         8

Formula: C1039H1736N324O321S8
Total number of atoms: 3428

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    11585
Abs 0.1% (=1 g/l)   0.480, assuming all pairs of Cys residues form cystines


Ext. coefficient    11460
Abs 0.1% (=1 g/l)   0.474, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 56.53
This classifies the protein as unstable.



Aliphatic index: 75.45

Grand average of hydropathicity (GRAVY): -0.935