ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MADFLLRPIK QRHRNEDKYV SVDAADGSVS KIEPIADFVI KTKLLSANGP EKLQDGRKVF 

        70         80         90        100        110        120 
INVCHSPLVP KPEVDFNARI VFPLIIQNEW EIPIITSCYR MDHDKKGQEC YVWDCCINSD 

       130        140        150        160        170        180 
CSRWICDDIQ LREILVEWCL ESCEIRDSVV LCRDRIAFPK MKKKGAELPA LEVLNDELHQ 

       190        200        210        220        230        240 
DYKAKMHKII EEEAGDPMSI LRGRNDDGDD NNDPDDGTLP PLFPIENKIS GAKIEEIDKN 

       250        260        270        280        290        300 
EIAHRNLKQA PAPAPAPHEQ QEDVPEYEVK MKRFKGAAYK LRILIENKAP NSKPDRFSPS 

       310        320        330        340 
YNFAENILYI NGKLSIPLPR DIVVNAADIK IFHIRKERTL YIYI 

References and documentation are available.

Number of amino acids: 344

Molecular weight: 39519.39

Theoretical pI: 5.63

Amino acid composition: 
Ala (A)  23	  6.7%
Arg (R)  20	  5.8%
Asn (N)  19	  5.5%
Asp (D)  30	  8.7%
Cys (C)  10	  2.9%
Gln (Q)   9	  2.6%
Glu (E)  28	  8.1%
Gly (G)  12	  3.5%
His (H)   8	  2.3%
Ile (I)  36	 10.5%
Leu (L)  25	  7.3%
Lys (K)  30	  8.7%
Met (M)   6	  1.7%
Phe (F)  11	  3.2%
Pro (P)  25	  7.3%
Ser (S)  16	  4.7%
Thr (T)   4	  1.2%
Trp (W)   4	  1.2%
Tyr (Y)  10	  2.9%
Val (V)  18	  5.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 58
Total number of positively charged residues (Arg + Lys): 50

Atomic composition:

Carbon      C	      1760
Hydrogen    H	      2790
Nitrogen    N	       482
Oxygen      O	       519
Sulfur      S	        16

Formula: C1760H2790N482O519S16
Total number of atoms: 5567

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    37525
Abs 0.1% (=1 g/l)   0.950, assuming all pairs of Cys residues form cystines


Ext. coefficient    36900
Abs 0.1% (=1 g/l)   0.934, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 56.03
This classifies the protein as unstable.



Aliphatic index: 91.02

Grand average of hydropathicity (GRAVY): -0.492