ProtParam
User-provided sequence:
10 20 30 40 50 60
MADGEDIQPL VCDNGTGMVK AGFAGDDAPR AVFPSIVGRP RHTGVMVGMG QKDAYVGDEA
70 80 90 100 110 120
QSKRGILTLK YPIEHGIVNN WDDMEKIWHH TFYNELRVAP EEHPVLLTEA PLNPKANREK
130 140 150 160 170 180
MTQIMFETFN TPAMYVAIQA VLSLYASGRT TGIVLDSGDG VSHTVPIYEG YALPHAILRL
190 200 210 220 230 240
DLAGRDLTDH LMKILTERGY SFTTTAEREI VRDMKEKLSY IALDFEQELE TSKTSSSVEK
250 260 270 280 290 300
SFELPDGQVI TIGAERFRCP EVLFQPSMIG MENPGIHETT YNSIMKCDVD IRKDLYGNIV
310 320 330 340 350 360
LSGGTTMFGG IGDRMSKEIT ALAPSSMKIK VVAPPERKYS VWIGGSILAS LSTFQQMWIA
370
KAEYDESGPS IVHRKCF
References and
documentation are available.
Number of amino acids: 377
Molecular weight: 41778.79
Theoretical pI: 5.37
Total number of negatively charged residues (Asp + Glu): 50
Total number of positively charged residues (Arg + Lys): 38
Atomic composition:
Carbon C 1853
Hydrogen H 2917
Nitrogen N 495
Oxygen O 561
Sulfur S 21
Formula: C
1853H
2917N
495O
561S
21
Total number of atoms: 5847
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 41620
Abs 0.1% (=1 g/l) 0.996, assuming all pairs of Cys residues form cystines
Ext. coefficient 41370
Abs 0.1% (=1 g/l) 0.990, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 34.61
This classifies the protein as stable.
Aliphatic index: 83.02
Grand average of hydropathicity (GRAVY): -0.211