ProtParam
User-provided sequence:
10 20 30 40 50 60
MADGEDIQPL VCDNGTGMVK AGFAGDDAPR AVFPSIVGRP RHTGVMVGMG QKDAYVGDEA
70 80 90 100 110 120
QSKRGILTLK YPIEHGIVSN WDDMEKIWHH TFYNELRVAP EEHPVLLTEA PLNPKANREK
130 140 150 160 170 180
MTQIMFETFN VPAMYVAIQA VLSLYASGRT TGIVLDSGDG VSHTVPIYEG YALPHAILRL
190 200 210 220 230 240
DLAGRDLTDS LMKILTERGY MFTTTAEREI VRDIKEKLAY VALDYEQELE TAKSSSSVEK
250 260 270 280 290 300
NYELPDGQVI TIGAERFRCP EVLFQPSLIG MEAPGIHETT YNSIMKCDVD IRKDLYGNIV
310 320 330 340 350 360
LSGGSTMFPG IADRMSKEIT ALAPSSMKIK VVAPPERKYS VWIGGSILAS LSTFQQMWIS
370
KSEYDESGPS IVHRKCF
References and
documentation are available.
Number of amino acids: 377
Molecular weight: 41735.78
Theoretical pI: 5.31
Total number of negatively charged residues (Asp + Glu): 50
Total number of positively charged residues (Arg + Lys): 38
Atomic composition:
Carbon C 1855
Hydrogen H 2924
Nitrogen N 492
Oxygen O 561
Sulfur S 20
Formula: C
1855H
2924N
492O
561S
20
Total number of atoms: 5852
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 44600
Abs 0.1% (=1 g/l) 1.069, assuming all pairs of Cys residues form cystines
Ext. coefficient 44350
Abs 0.1% (=1 g/l) 1.063, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.39
This classifies the protein as stable.
Aliphatic index: 86.13
Grand average of hydropathicity (GRAVY): -0.179