ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MADHKGKVPV SLSASSSSLS LSPKTTPIEA EVWLIAEARA QEILCAIQPN YLAERSRNKI 

        70         80         90        100        110        120 
ISNLQTLLWE RLGIEVYLFG SMPLKTYLPD GDIDLTVLTH HASEEDCARA VCCVLEAEMG 

       130        140        150        160        170        180 
NSDLQVTGVQ YVQAKVKVIK CSIRDVAFDI SFNQLAGLGA LCFLEQVDKA FGRDHLFKKS 

       190        200        210        220        230        240 
IILVKAWCFY ESRILGANSG LISTYALAIL VLNIVNMSYS SLSGPLAVLY KFINYYGSFD 

       250        260        270        280        290        300 
WKNYCVTVTG PVPISSLPDI TETGNHEVFL DEKFFRECME LYSGETGVVE ASRKYFPVKY 

       310        320        330        340        350        360 
YNILDPLKHS NNLGRSVTKG NMVRLRNCFM LGVQKLRDVL TLPGENVGWK LEKFFNVSLE 

       370        380        390        400        410        420 
RNGKGQRQDV EEPVVAFGTG AADYSQLKGD FDRYRNNLIY GKWFHGESLH NWLPPSLDTT 

       430        440        450        460        470        480 
SWANISFYMS RVRNGFNGRN QEGPTSMPNI KQSPNNRRSN GTGTYIPGMS KQSNTAGCSS 

       490        500 
SKPSTVKSLP SASETQESKK NP 

References and documentation are available.

Number of amino acids: 502

Molecular weight: 55903.77

Theoretical pI: 8.48

Amino acid composition: 
Ala (A)  29	  5.8%
Arg (R)  23	  4.6%
Asn (N)  32	  6.4%
Asp (D)  20	  4.0%
Cys (C)  11	  2.2%
Gln (Q)  16	  3.2%
Glu (E)  29	  5.8%
Gly (G)  36	  7.2%
His (H)   8	  1.6%
Ile (I)  25	  5.0%
Leu (L)  50	 10.0%
Lys (K)  31	  6.2%
Met (M)  10	  2.0%
Phe (F)  21	  4.2%
Pro (P)  23	  4.6%
Ser (S)  50	 10.0%
Thr (T)  24	  4.8%
Trp (W)   8	  1.6%
Tyr (Y)  20	  4.0%
Val (V)  36	  7.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 54

Atomic composition:

Carbon      C	      2495
Hydrogen    H	      3904
Nitrogen    N	       674
Oxygen      O	       743
Sulfur      S	        21

Formula: C2495H3904N674O743S21
Total number of atoms: 7837

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    74425
Abs 0.1% (=1 g/l)   1.331, assuming all pairs of Cys residues form cystines


Ext. coefficient    73800
Abs 0.1% (=1 g/l)   1.320, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.55
This classifies the protein as unstable.



Aliphatic index: 84.84

Grand average of hydropathicity (GRAVY): -0.238