ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MADITDKTAE QLENLNIQDD QKQAATGSES QSVENSSASL YVGDLEPSVS EAHLYDIFSP 

        70         80         90        100        110        120 
IGSVSSIRVC RDAITKTSLG YAYVNFNDHE AGRKAIEQLN YTPIKGRLCR IMWSQRDPSL 

       130        140        150        160        170        180 
RKKGSGNIFI KNLHPDIDNK ALYDTFSVFG DILSSKIATD ENGKSKGFGF VHFEEEGAAK 

       190        200        210        220        230        240 
EAIDALNGML LNGQEIYVAP HLSRKERDSQ LEETKAHYTN LYVKNINSET TDEQFQELFA 

       250        260        270        280        290        300 
KFGPIVSASL EKDADGKLKG FGFVNYEKHE DAVKAVEALN DSELNGEKLY VGRAQKKNER 

       310        320        330        340        350        360 
MHVLKKQYEA YRLEKMAKYQ GVNLFVKNLD DSVDDEKLEE EFAPYGTITS AKVMRTENGK 

       370        380        390        400        410        420 
SKGFGFVCFS TPEEATKAIT EKNQQIVAGK PLYVAIAQRK DVRRSQLAQQ IQARNQMRYQ 

       430        440        450        460        470        480 
QATAAAAAAA AGMPGQFMPP MFYGVMPPRG VPFNGPNPQQ MNPMGGMPKN GMPPQFRNGP 

       490        500        510        520        530        540 
VYGVPPQGGF PRNANDNNQF YQQKQRQALG EQLYKKVSAK TSNEEAAGKI TGMILDLPPQ 

       550        560        570 
EVFPLLESDE LFEQHYKEAS AAYESFKKEQ EQQTEQA 

References and documentation are available.

Number of amino acids: 577

Molecular weight: 64344.20

Theoretical pI: 5.71

Amino acid composition: 
Ala (A)  54	  9.4%
Arg (R)  22	  3.8%
Asn (N)  34	  5.9%
Asp (D)  29	  5.0%
Cys (C)   3	  0.5%
Gln (Q)  41	  7.1%
Glu (E)  49	  8.5%
Gly (G)  42	  7.3%
His (H)   9	  1.6%
Ile (I)  25	  4.3%
Leu (L)  38	  6.6%
Lys (K)  47	  8.1%
Met (M)  16	  2.8%
Phe (F)  27	  4.7%
Pro (P)  29	  5.0%
Ser (S)  36	  6.2%
Thr (T)  22	  3.8%
Trp (W)   1	  0.2%
Tyr (Y)  23	  4.0%
Val (V)  30	  5.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 78
Total number of positively charged residues (Arg + Lys): 69

Atomic composition:

Carbon      C	      2835
Hydrogen    H	      4428
Nitrogen    N	       784
Oxygen      O	       890
Sulfur      S	        19

Formula: C2835H4428N784O890S19
Total number of atoms: 8956

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    39895
Abs 0.1% (=1 g/l)   0.620, assuming all pairs of Cys residues form cystines


Ext. coefficient    39770
Abs 0.1% (=1 g/l)   0.618, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.18
This classifies the protein as unstable.



Aliphatic index: 67.02

Grand average of hydropathicity (GRAVY): -0.678