ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MADKSETTPP SIDGNVLVAK SLSHLGVTHM FGVVGIPVTS LASRAMALGI RFIAFHNEQS 

        70         80         90        100        110        120 
AGYAASAYGY LTGKPGILLT VSGPGCVHGL AGLSNAWVNT WPMVMISGSC DQRDVGRGDF 

       130        140        150        160        170        180 
QELDQIEAVK AFSKLSEKAK DVREIPDCVS RVLDRAVSGR PGGCYLDIPT DVLRQKISES 

       190        200        210        220        230        240 
EADKLVDEVE RSRKEEPIRG SLRSEIESAV SLLRKAERPL IVFGKGAAYS RAEDELKKLV 

       250        260        270        280        290        300 
EITGIPFLPT PMGKGLLPDT HEFSATAARS LAIGKCDVAL VVGARLNWLL HFGESPKWDK 

       310        320        330        340        350        360 
DVKFILVDVS EEEIELRKPH LGIVGDAKTV IGLLNREIKD DPFCLGKSNS WVESISKKAK 

       370        380        390        400        410        420 
ENGEKMEIQL AKDVVPFNFL TPMRIIRDAI LAVEGPSPVV VSEGANTMDV GRSVLVQKEP 

       430        440        450        460        470        480 
RTRLDAGTWG TMGVGLGYCI AAAVASPDRL VVAVEGDSGF GFSAMEVETL VRYNLAVVII 

       490        500        510        520        530        540 
VFNNGGVYGG DRRGPEEISG PHKEDPAPTS FVPNAGYHKL IEAFGGKGYI VETPDELKSA 

       550        560        570 
LAESFAARKP AVVNVIIDPF AGAESGRLQH KN 

References and documentation are available.

Number of amino acids: 572

Molecular weight: 61470.47

Theoretical pI: 5.74

Amino acid composition: 
Ala (A)  52	  9.1%
Arg (R)  31	  5.4%
Asn (N)  16	  2.8%
Asp (D)  32	  5.6%
Cys (C)   7	  1.2%
Gln (Q)   8	  1.4%
Glu (E)  43	  7.5%
Gly (G)  56	  9.8%
His (H)  10	  1.7%
Ile (I)  34	  5.9%
Leu (L)  49	  8.6%
Lys (K)  35	  6.1%
Met (M)  11	  1.9%
Phe (F)  20	  3.5%
Pro (P)  32	  5.6%
Ser (S)  43	  7.5%
Thr (T)  21	  3.7%
Trp (W)   6	  1.0%
Tyr (Y)  10	  1.7%
Val (V)  56	  9.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 75
Total number of positively charged residues (Arg + Lys): 66

Atomic composition:

Carbon      C	      2734
Hydrogen    H	      4380
Nitrogen    N	       750
Oxygen      O	       821
Sulfur      S	        18

Formula: C2734H4380N750O821S18
Total number of atoms: 8703

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    48275
Abs 0.1% (=1 g/l)   0.785, assuming all pairs of Cys residues form cystines


Ext. coefficient    47900
Abs 0.1% (=1 g/l)   0.779, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.26
This classifies the protein as stable.



Aliphatic index: 94.07

Grand average of hydropathicity (GRAVY): -0.058