ProtParam
User-provided sequence:
10 20 30 40 50 60
MADKVLRAKR KQFINSVSVG TINGLLDELL EKRVLNQEEM DTIKLANITV MEKARDLCDH
70 80 90 100 110 120
VTKKGPRASQ MFITYICNED CYLAEILELQ SGPSAETVFV TEDSKGGHPF SSETKEKLNK
130 140 150 160 170 180
EGGAFPGPSG SLKFCPLEIA QKLWKENHSE IYPIMKTPTR TRLALIICST DFQHLSRRVG
190 200 210 220 230 240
ADVDLREMKL LLQDLGYTVK VKENLTALEM TKELKEFAAC PEHKTSDSTF LVFMSHGLQE
250 260 270 280 290 300
GICGITYSNE VADILKVDTI FQMMNTLKCP SLKDKPKVII IQACRGEKQG VVLLKDSVGN
310 320 330 340 350 360
SEEGFLTDAI FEDDGIKKAH IEKDFIAFCS STPDNVSWRH PVRGSLFIES LIKHMKEYAW
370 380 390 400
SCDLEDIFRK VRFSFEQPDS RLQMPTTERV TLTKRFYLFP GH
References and
documentation are available.
Number of amino acids: 402
Molecular weight: 45548.56
Theoretical pI: 6.25
Total number of negatively charged residues (Asp + Glu): 58
Total number of positively charged residues (Arg + Lys): 54
Atomic composition:
Carbon C 2024
Hydrogen H 3232
Nitrogen N 540
Oxygen O 605
Sulfur S 23
Formula: C
2024H
3232N
540O
605S
23
Total number of atoms: 6424
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 27555
Abs 0.1% (=1 g/l) 0.605, assuming all pairs of Cys residues form cystines
Ext. coefficient 26930
Abs 0.1% (=1 g/l) 0.591, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 43.01
This classifies the protein as unstable.
Aliphatic index: 85.60
Grand average of hydropathicity (GRAVY): -0.299