ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MADLFAPAPE PSTELGRLRV LSKSAGIRVS PLILGGMSIG DAWSEILGSM SKERAFELLD 

        70         80         90        100        110        120 
AFYEAGGNFI DTANNYQNEQ SEAWIGEWMV SRKLRDQIVI ATKFTTDYKK YDVGGGKSAN 

       130        140        150        160        170        180 
YCGNHKRSLH VSVRDSLRKL QTDWIDILYV HWWDYMSSIE EVMDSLHILV QQARSSIWVC 

       190        200        210 
LIRLPGLFLR QITTLNLMVK PLLASIKVNG TC 

References and documentation are available.

Number of amino acids: 212

Molecular weight: 23918.56

Theoretical pI: 6.90

Amino acid composition: 
Ala (A)  14	  6.6%
Arg (R)  12	  5.7%
Asn (N)   8	  3.8%
Asp (D)  12	  5.7%
Cys (C)   3	  1.4%
Gln (Q)   7	  3.3%
Glu (E)  11	  5.2%
Gly (G)  15	  7.1%
His (H)   4	  1.9%
Ile (I)  16	  7.5%
Leu (L)  24	 11.3%
Lys (K)  11	  5.2%
Met (M)   7	  3.3%
Phe (F)   6	  2.8%
Pro (P)   6	  2.8%
Ser (S)  20	  9.4%
Thr (T)   9	  4.2%
Trp (W)   7	  3.3%
Tyr (Y)   7	  3.3%
Val (V)  13	  6.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 23
Total number of positively charged residues (Arg + Lys): 23

Atomic composition:

Carbon      C	      1073
Hydrogen    H	      1689
Nitrogen    N	       289
Oxygen      O	       310
Sulfur      S	        10

Formula: C1073H1689N289O310S10
Total number of atoms: 3371

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    49055
Abs 0.1% (=1 g/l)   2.051, assuming all pairs of Cys residues form cystines


Ext. coefficient    48930
Abs 0.1% (=1 g/l)   2.046, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 59.06
This classifies the protein as unstable.



Aliphatic index: 97.97

Grand average of hydropathicity (GRAVY): -0.073