ProtParam
User-provided sequence:
10 20 30 40 50 60
MADQLNNEPG QPTPLMSIRF DQQQHESAAA NGNENGNGNA NGDGDASEDA VDAEKTPGEL
70 80 90 100 110 120
VLPKALEDVL ALKDQRAAEF TVDADSQGGS GGATQDGDEA DVGEDSEEDA DNQVNGSASQ
130 140 150 160 170 180
KTGKQSKAER NKKKKKRKKQ NRKIRHQIEF ARQQQLAQAE ENEPEAKDSE TKAHGSEDEQ
190 200 210 220 230 240
VAEKEKKDKR KESRSKRRKD RSDEKDTDKK AGEANDENVT IEYVPEKITI ADLAPMYRQF
250 260 270 280 290 300
YRVFEIFKLE NKPKPVEKDK SSHDSESHPR DKKATDKQLE DEDDDGDDDE ERKEDKEKLS
310 320 330 340 350 360
KRKLKKLTRL SVAELKQLVS RPDVVEMHDV TARDPKLLVQ LKAYRNTVQV PRHWCFKRKY
370 380 390 400 410 420
LQGKRGIEKP PFDLPAFIKK TGIMEMRESL QEREDAKTLK AKMRERVRPK MGKIDIDYQK
430 440 450 460 470 480
LHDAFFKWQT KPRMTIHGDL YYEGKEFETR LKEKKPGDLS EELRIALGMP VGPNSHKIPP
490 500 510 520 530 540
PWLIAQQRYG PPPSYPNLKI PGLNAPIPDG TSFGYHAGGW GKPPVDENGK PLYGDVFGTN
550 560 570 580 590 600
ILDLDNGVDE ADIERNQWGE LESESEESSE EEEEDGEDLG DQQDETGLVT PVEGLVTPSG
610 620 630 640 650 660
LTSVPAGMET PENIELRKKK IEAEMEDNET PVLYQVLPEK RTDRIGASMM GSTHVYDVSG
670 680 690 700 710 720
SGANKQPPVR STTDREGIVE LALDPSELDL DNDAMAQRYE QQMREQQNHL QKEDLSDMLA
730 740
EHVARQKSKR KRQQTDPAKT TKKYKEFKF
References and
documentation are available.
Number of amino acids: 749
Molecular weight: 84781.08
Theoretical pI: 5.21
Total number of negatively charged residues (Asp + Glu): 154
Total number of positively charged residues (Arg + Lys): 125
Atomic composition:
Carbon C 3652
Hydrogen H 5845
Nitrogen N 1067
Oxygen O 1219
Sulfur S 18
Formula: C
3652H
5845N
1067O
1219S
18
Total number of atoms: 11801
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 51340
Abs 0.1% (=1 g/l) 0.606, assuming all pairs of Cys residues form cystines
Ext. coefficient 51340
Abs 0.1% (=1 g/l) 0.606, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 46.53
This classifies the protein as unstable.
Aliphatic index: 59.41
Grand average of hydropathicity (GRAVY): -1.204