ProtParam
User-provided sequence:
10 20 30 40 50 60
MADVDDFDSL SNAELRAKML AQGLPNIPVT DSSRKVLVKR LRASIGGQAS PAASPKKTNR
70 80 90 100 110 120
RETLAPAPGA PSAPAAASTP VDKLDGNKVA PATKARRTIT AAEAKEPVRR LPEEAIRRRP
130 140 150 160 170 180
DEADRLRSEE PVAARKPTTA PAAQPVQTRR TSTSSGSERK VVEPLRKPET IVEQPASSKR
190 200 210 220 230 240
ADREENYLKV NSLIVLESDE EEDEQLVQAA DLVEQEHAAR QKTTKLASSG TTTYEYKSKV
250 260 270 280 290 300
VEPPRRQVYE ATAAPVLPPS VPSARAQTTS STRSYDYASN PAPGRYSSFV RTAAQGYVTA
310 320 330 340 350 360
EAPPVASYSS SYKRTYANEL SDDTDSKEDQ YESTFARNLA RLRAERIGDR ISPYSRRTLA
370 380 390 400 410 420
SGNAGSGSLG YEPRARRSLR PNDNSVSEAF NRWLNSLEQK YHIKSKLFIV LLVLLLIGVY
YIFY
References and
documentation are available.
Number of amino acids: 424
Molecular weight: 46584.10
Theoretical pI: 9.56
Total number of negatively charged residues (Asp + Glu): 53
Total number of positively charged residues (Arg + Lys): 65
Atomic composition:
Carbon C 2024
Hydrogen H 3280
Nitrogen N 606
Oxygen O 651
Sulfur S 2
Formula: C
2024H
3280N
606O
651S
2
Total number of atoms: 6563
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 32320
Abs 0.1% (=1 g/l) 0.694
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 68.71
This classifies the protein as unstable.
Aliphatic index: 72.59
Grand average of hydropathicity (GRAVY): -0.696