ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MADVHKGKVC VTGAGGFLGS WVVDLLLSKD YFVHGTVRDP DNEKYAHLKK LEKAGDKLKL 

        70         80         90        100        110        120 
FKADLLDYGS LQSAIAGCSG VFHVACPVPP ASVPNPEVEL IAPAVDGTLN VLKACIEANV 

       130        140        150        160        170        180 
KRVVYVSSVA AAFMNPMWSK NQVLDEACWS DQEYCKKTEN WYCLAKTRAE SEAFEFAKRT 

       190        200        210        220        230        240 
GLHLVSVCPT LVLGPILQQN TVNASSLVLL KLLKEGFETR DNQERHLVDV RDVAQALLLV 

       250        260        270        280        290        300 
YEKAEAEGRY ICTSHTVKEE IVVEKLKSFY PHYNYPKKYI DAEDRVKVSS EKLQKLGWTY 

       310        320 
RPLEETLVDS VESYRKAKLV D 

References and documentation are available.

Number of amino acids: 321

Molecular weight: 35875.29

Theoretical pI: 6.47

Amino acid composition: 
Ala (A)  28	  8.7%
Arg (R)  11	  3.4%
Asn (N)  11	  3.4%
Asp (D)  18	  5.6%
Cys (C)   9	  2.8%
Gln (Q)   8	  2.5%
Glu (E)  25	  7.8%
Gly (G)  16	  5.0%
His (H)   8	  2.5%
Ile (I)   7	  2.2%
Leu (L)  36	 11.2%
Lys (K)  30	  9.3%
Met (M)   3	  0.9%
Phe (F)   9	  2.8%
Pro (P)  13	  4.0%
Ser (S)  20	  6.2%
Thr (T)  13	  4.0%
Trp (W)   5	  1.6%
Tyr (Y)  14	  4.4%
Val (V)  37	 11.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 43
Total number of positively charged residues (Arg + Lys): 41

Atomic composition:

Carbon      C	      1615
Hydrogen    H	      2550
Nitrogen    N	       424
Oxygen      O	       474
Sulfur      S	        12

Formula: C1615H2550N424O474S12
Total number of atoms: 5075

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    48860
Abs 0.1% (=1 g/l)   1.362, assuming all pairs of Cys residues form cystines


Ext. coefficient    48360
Abs 0.1% (=1 g/l)   1.348, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 31.49
This classifies the protein as stable.



Aliphatic index: 94.39

Grand average of hydropathicity (GRAVY): -0.176