ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MADVKVQSES ESSDSHPLVD YQSLPPYPPP HPPVEVEENQ PKTSPTPPPP HWMRYPPVLM 

        70         80         90        100        110        120 
PQMMYAPPPP MPFSPYHQYP NHHHFHHQSR GNKHQNAFNG ENKTIWVGDL QNWMDEAYLN 

       130        140        150        160        170        180 
SAFTSAEERE IVSLKVIRNK HNGSSEGYGF VEFESHDVAD KVLQEFNGAP MPNTDQPFRL 

       190        200        210        220        230        240 
NWASFSTGEK RLENNGPDLS IFVGDLAPDV SDALLHETFS EKYPSVKAAK VVLDANTGRS 

       250        260        270        280        290        300 
KGYGFVRFGD ENERTKAMTE MNGVKCSSRA MRIGPATPRK TNGYQQQGGY MPSGAFTRSE 

       310        320        330        340        350        360 
GDTINTTIFV GGLDSSVTDE DLKQPFSEFG EIVSVKIPVG KGCGFVQFVN RPNAEEALEK 

       370        380        390        400        410        420 
LNGTVIGKQT VRLSWGRNPA NKQPRDKYGN QWVDPYYGGQ FYNGYGYMVP QPDPRMYPAA 

       430 
PYYPMYGGHQ QQVS 

References and documentation are available.

Number of amino acids: 434

Molecular weight: 48632.17

Theoretical pI: 5.88

Amino acid composition: 
Ala (A)  23	  5.3%
Arg (R)  18	  4.1%
Asn (N)  27	  6.2%
Asp (D)  21	  4.8%
Cys (C)   2	  0.5%
Gln (Q)  23	  5.3%
Glu (E)  28	  6.5%
Gly (G)  37	  8.5%
His (H)  14	  3.2%
Ile (I)  10	  2.3%
Leu (L)  19	  4.4%
Lys (K)  22	  5.1%
Met (M)  15	  3.5%
Phe (F)  20	  4.6%
Pro (P)  45	 10.4%
Ser (S)  32	  7.4%
Thr (T)  19	  4.4%
Trp (W)   6	  1.4%
Tyr (Y)  22	  5.1%
Val (V)  31	  7.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 40

Atomic composition:

Carbon      C	      2165
Hydrogen    H	      3242
Nitrogen    N	       594
Oxygen      O	       656
Sulfur      S	        17

Formula: C2165H3242N594O656S17
Total number of atoms: 6674

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    65905
Abs 0.1% (=1 g/l)   1.355, assuming all pairs of Cys residues form cystines


Ext. coefficient    65780
Abs 0.1% (=1 g/l)   1.353, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 49.16
This classifies the protein as unstable.



Aliphatic index: 52.07

Grand average of hydropathicity (GRAVY): -0.782