ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MADVNLPPSD SVDNRLPEKS TSSSPPPPPP SSSLPQQEQE QDQQQLPLRR ERDSRERRDE 

        70         80         90        100        110        120 
RDIERPPPNR RERDRSPLPP PRRDYKRRPS LSPPPPYRDR RHSPPQRRSP PQKRYRRDDN 

       130        140        150        160        170        180 
GYDGRRGSPR GGYGPPDRRF GYDHGGGYDR EMGGRPGYGD ERPHGRFMGR YQDWEGGRGG 

       190        200        210        220        230        240 
YGDASNSGNP QRDGLMSYKQ FIQELEDDIL PSEAERRYQE YKSEYITTQK RAFFNTHKEE 

       250        260        270        280        290        300 
DWLKNKYHPT NLLSVIERRN DLAQKVAKDF LLDLQSGTLD LGPAVTALNK SGRTSEPNSE 

       310        320        330        340        350        360 
DEAAGVGKRK RHGMGGAKEN ELLSAAPKAP SFTSDPKRIL TDVEQTQALV RKLDSEKKIE 

       370        380        390        400        410        420 
ENVLQGSETE KSGREKLHSG STGPVVIIRG LTSVKGLEGV ELLDTLVTYL WRVHGLDYYG 

       430        440        450        460        470        480 
KVETNEAKGL RHVRAEGKVS DAKGDENESK FDSHWQERLK GQDPLEVMAA KEKIDAAATE 

       490        500        510        520        530        540 
ALDPHVRKIR DEKYGWKYGC GAKGCTKLFH AAEFVYKHLK LKHTELVTEL TTKVREELYF 

       550        560        570        580        590        600 
QNYMNDPNAP GGQPATQQSG PRDRPIRRKP SMENRLRDDR GGRRERDGRA NGNDRNDRSE 

       610        620        630        640        650        660 
DQQRGDNDGG NPGEVGYDAF GGQGGVHVPP FLSDINPPPM LMPVPGAGPL GPFVPAPPEV 

       670        680        690        700        710        720 
AMQMFRDPSG PNPPFEGSGR GGPAPFLLSP AFRQDPRRLR SYQDLDAPEE EVTVIDYRSL 

References and documentation are available.

Number of amino acids: 720

Molecular weight: 81097.88

Theoretical pI: 8.41

Amino acid composition: 
Ala (A)  37	  5.1%
Arg (R)  77	 10.7%
Asn (N)  27	  3.8%
Asp (D)  57	  7.9%
Cys (C)   2	  0.3%
Gln (Q)  32	  4.4%
Glu (E)  58	  8.1%
Gly (G)  72	 10.0%
His (H)  15	  2.1%
Ile (I)  14	  1.9%
Leu (L)  53	  7.4%
Lys (K)  41	  5.7%
Met (M)  12	  1.7%
Phe (F)  18	  2.5%
Pro (P)  71	  9.9%
Ser (S)  47	  6.5%
Thr (T)  25	  3.5%
Trp (W)   5	  0.7%
Tyr (Y)  26	  3.6%
Val (V)  31	  4.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 115
Total number of positively charged residues (Arg + Lys): 118

Atomic composition:

Carbon      C	      3509
Hydrogen    H	      5520
Nitrogen    N	      1086
Oxygen      O	      1108
Sulfur      S	        14

Formula: C3509H5520N1086O1108S14
Total number of atoms: 11237

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    66365
Abs 0.1% (=1 g/l)   0.818, assuming all pairs of Cys residues form cystines


Ext. coefficient    66240
Abs 0.1% (=1 g/l)   0.817, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 66.89
This classifies the protein as unstable.



Aliphatic index: 53.92

Grand average of hydropathicity (GRAVY): -1.194