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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MADYSSLTVV QLKDLLTKRN LSVGGLKNEL VQRLIKDDEE SKGESEVSPQ EQNQEQGSEP

70 80 90 100 110 120
AAIEEPASQN ITEKKEVSSE PKETNEPKEE NKDVQKPSDG PSATASENEQ AAASTAAPAL

130 140 150 160 170 180
SPEEIKAKAL DLLNKKLHRA NKFGQDQADI DSLQRQINRV EKFGVDLNSK LAEELGLVSR

190 200 210
KNEPESGNNG KFKNRNKNAN NRSRVSKNRR GNRSGYRR


References and documentation are available.

Number of amino acids: 218 Molecular weight: 24137.56 Theoretical pI: 6.69
Amino acid composition: 
Ala (A) 18 8.3% Arg (R) 14 6.4% Asn (N) 21 9.6% Asp (D) 11 5.0% Cys (C) 0 0.0% Gln (Q) 13 6.0% Glu (E) 26 11.9% Gly (G) 12 5.5% His (H) 1 0.5% Ile (I) 6 2.8% Leu (L) 18 8.3% Lys (K) 23 10.6% Met (M) 1 0.5% Phe (F) 3 1.4% Pro (P) 10 4.6% Ser (S) 22 10.1% Thr (T) 6 2.8% Trp (W) 0 0.0% Tyr (Y) 2 0.9% Val (V) 11 5.0% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 37 Total number of positively charged residues (Arg + Lys): 37 Atomic composition: Carbon C 1018 Hydrogen H 1685 Nitrogen N 319 Oxygen O 357 Sulfur S 1 Formula: C1018H1685N319O357S1 Total number of atoms: 3380 Extinction coefficients: This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 2980 Abs 0.1% (=1 g/l) 0.123 Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 65.60 This classifies the protein as unstable. Aliphatic index: 65.83 Grand average of hydropathicity (GRAVY): -1.217