ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAEARTSRWY FGGLASCGAA CCTHPLDLLK VHLQTQQEVK LRMTGMALQV VRTDGFLALY 

        70         80         90        100        110        120 
NGLSASLCRQ MTYSLTRFAI YETMRDYMTK DSQGPLPFYS KVLLGGISGL TGGFVGTPAD 

       130        140        150        160        170        180 
LVNVRMQNDM KLPLSQRRNY SHALDGLYRV AREEGLKKLF SGATMASSRG ALVTVGQLSC 

       190        200        210        220        230        240 
YDQAKQLVLS TGYLSDNIFT HFLSSFIAGG CATFLCQPLD VLKTRLMNSK GEYQGVFHCA 

       250        260        270        280 
VETAKLGPQA FFKGLVPAGV RLVPHTVLTF MFLEQLRKHF GIKVAT 

References and documentation are available.

Number of amino acids: 286

Molecular weight: 31454.66

Theoretical pI: 9.42

Amino acid composition: 
Ala (A)  23	  8.0%
Arg (R)  16	  5.6%
Asn (N)   6	  2.1%
Asp (D)  10	  3.5%
Cys (C)   8	  2.8%
Gln (Q)  15	  5.2%
Glu (E)   8	  2.8%
Gly (G)  27	  9.4%
His (H)   7	  2.4%
Ile (I)   5	  1.7%
Leu (L)  39	 13.6%
Lys (K)  14	  4.9%
Met (M)  11	  3.8%
Phe (F)  16	  5.6%
Pro (P)   9	  3.1%
Ser (S)  19	  6.6%
Thr (T)  21	  7.3%
Trp (W)   1	  0.3%
Tyr (Y)  11	  3.8%
Val (V)  20	  7.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 18
Total number of positively charged residues (Arg + Lys): 30

Atomic composition:

Carbon      C	      1407
Hydrogen    H	      2230
Nitrogen    N	       384
Oxygen      O	       395
Sulfur      S	        19

Formula: C1407H2230N384O395S19
Total number of atoms: 4435

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    22390
Abs 0.1% (=1 g/l)   0.712, assuming all pairs of Cys residues form cystines


Ext. coefficient    21890
Abs 0.1% (=1 g/l)   0.696, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.05
This classifies the protein as stable.



Aliphatic index: 88.32

Grand average of hydropathicity (GRAVY): 0.091