ProtParam
User-provided sequence:
10 20 30 40 50 60
MAEASIEKTQ ILQKYLELDQ RGRIIAEYVW IDGTGNLRSK GRTLKKRITS IDQLPEWNFD
70 80 90 100 110 120
GSSTNQAPGH DSDIYLKPVA YYPDPFRRGD NIVVLAACYN NDGTPNKFNH RHEAAKLFAA
130 140 150 160 170 180
HKDEEIWFGL EQEYTLFDMY DDVYGWPKGG YPAPQGPYYC GVGAGKVYAR DMIEAHYRAC
190 200 210 220 230 240
LYAGLEISGI NAEVMPSQWE FQVGPCTGID MGDQLWMARY FLHRVAEEFG IKISFHPKPL
250 260 270 280 290 300
KGDWNGAGCH TNVSTKEMRQ PGGMKYIEQA IEKLSKRHAE HIKLYGSDND MRLTGRHETA
310 320 330 340 350 360
SMTAFSSGVA NRGSSIRIPR SVAKEGYGYF EDRRPASNID PYLVTGIMCE TVCGAIDNAD
370
MTKEFERESS
References and
documentation are available.
Number of amino acids: 370
Molecular weight: 41766.00
Theoretical pI: 5.92
Total number of negatively charged residues (Asp + Glu): 50
Total number of positively charged residues (Arg + Lys): 43
Atomic composition:
Carbon C 1851
Hydrogen H 2821
Nitrogen N 513
Oxygen O 556
Sulfur S 19
Formula: C
1851H
2821N
513O
556S
19
Total number of atoms: 5760
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 70165
Abs 0.1% (=1 g/l) 1.680, assuming all pairs of Cys residues form cystines
Ext. coefficient 69790
Abs 0.1% (=1 g/l) 1.671, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 44.35
This classifies the protein as unstable.
Aliphatic index: 66.24
Grand average of hydropathicity (GRAVY): -0.574